Calcium fluorescence recordings from neuroepithelial stem cells

Calcium fluorescence recordings from neuroepithelial stem cells
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神经上皮干细胞的钙荧光记录

DOI:
10.1007/7651_2020_290
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发表时间:
2020
期刊:
Methods in Molecular Biology, Springer Nature
影响因子:
--
通讯作者:
M.
M.
中科院分区:
--
文献类型:
--
作者:
Yamashita;M.

文献摘要

相似文献

神经上皮细胞在胚胎发育过程中通过自我更新发挥神经干细胞的作用。这些细胞紧密相连,并与神经上皮的基底膜接触。在这种情况下,Ca2+荧光记录是研究神经上皮干细胞生理特性的一种成功方法。本章描述了神经上皮干细胞Ca2+荧光记录的详细技术。
Neuroepithelial cells act as neural stem cells by renewing themselves during embryonic development. These cells are tightly interconnected and make contact with the basement membrane of the neuroepithelium. Under such circumstances, Ca2+fluorescence recording is a successful method to study physiological properties of the neuroepithelial stem cell. This chapter describes detailed techniques of Ca2+fluorescence recording from neuroepithelial stem cells.