Translin recognition site sequences flank chromosome translocation breakpoints in alveolar rhabdomyosarcoma cell lines.
Translin recognition site sequences flank chromosome translocation breakpoints in alveolar rhabdomyosarcoma cell lines.
复制标题
肺泡横纹肌肉瘤细胞系中易位蛋白识别位点序列位于染色体易位断点侧翼。
DOI：
10.1038/sj.onc.1201285
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发表时间：
1997
期刊：
影响因子：
8
通讯作者：
Mitchell,CD
中科院分区：
文献类型：
--
作者：
Chalk,JG;Barr,FG;Mitchell,CD
腺泡状横纹肌肉瘤的特征是at（2; 13）（q35; q14）染色体易位，导致PAX 3和FKHR基因融合。所得融合基因编码具有异常转录活性的嵌合蛋白。我们报告的分子定义的基因组断裂点的两个衍生染色体在一种情况下，衍生染色体13断裂点在另外两种情况下。与衍生染色体13上的断裂点相邻的DNA序列与蛋白质translin的识别序列相似。凝胶位移分析证实，这些序列结合translin。这些研究结果表明，translin可能不仅是重要的淋巴系统恶性肿瘤的染色体易位的起源，但也在实体瘤中发现的易位。
Alveolar rhabdomyosarcoma is characterized by at (2; 13)(q35; q14) chromosome translocation, which leads to the fusion of the PAX3 and the FKHR genes. The resulting fusion gene encodes a chimeric protein which has aberrant transcriptional activity. We report the molecular definition of the genomic breakpoints on both derivative chromosomes in one case and the derivative chromosome 13 breakpoints in two other cases. The DNA sequences adjacent to the breakpoints on the derivative chromosome 13 are remarkable for their resemblance to recognition sequences for the protein translin. Gel shift analyses confirm that these sequences bind translin. These findings suggest that translin may not only be important in the genesis of chromosomal translocations in lymphoid malignancy, but also in translocations found in solid tumours.