Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry
Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry
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DOI：
10.1002/rcm.2962
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Jaworski, Jan G.
中科院分区：
文献类型：
--
作者：
Markham, Jonathan E.;Jaworski, Jan G.
鞘脂的变化对植物和动物的细胞命运和发育有着深远的影响。鞘脂作为一个群体由大量不同的化合物类别组成，其中许多单独的物种可能会对环境刺激产生反应，从而影响细胞反应。因此，同时测量所有鞘脂的能力对于理解鞘脂代谢的生化调节及其衍生的信号分子至关重要。在模式植物拟南芥中，鞘脂类主要有糖基神经酰胺、糖基神经酰胺、羟基神经酰胺和神经酰胺。其他次要但潜在重要的鞘脂是游离的长链碱基及其磷酸化衍生物。通过使用反相高效液相色谱-电喷雾电离串联质谱检测的单溶剂系统，我们已经能够从原油样品中分离和测量168种鞘脂。这大大加快和简化了植物鞘脂的分析，并为更好地理解它们在植物性能中的作用铺平了道路。版权所有(c) 2007约翰威利父子有限公司
Changes in sphingolipids have been associated with profound effects in cell fate and development in both plants and animals. Sphingolipids as a group consist of a large number of different compound classes of which numerous individual species may vary in response to environmental stimuli to affect cellular responses. The ability to measure all sphingolipids simultaneously is, therefore, essential to an understanding of the biochemical regulation of sphingolipid metabolism and signaling molecules derived from it. In the model plant Arabidopsis thaliana, the major sphingolipid classes are glycosylinositolphosphoceramides, glucosylceramides, hydroxyceramides and ceramides. Other minor but potentially important sphingolipids are free long-chain bases and their phosphorylated derivates. By using a single solvent system with reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry detection we have been able to separate and measure 168 sphingolipids from a crude sample. This greatly speeds up and simplifies the analysis of plant sphingolipids and should pave the way for a better understanding of their role in plant performance. Copyright (c) 2007 John Wiley & Sons, Ltd.