Association of a single, nucleotide polymorphism in SPP1 with growth traits and twinning in cattle population selected for twinning rate
Association of a single, nucleotide polymorphism in SPP1 with growth traits and twinning in cattle population selected for twinning rate
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DOI：
10.2527/jas.2006-460
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发表时间：
2007-02-01
影响因子：
3.3
通讯作者：
Smith, T. P. L.
中科院分区：
文献类型：
--
作者：
Allan, M. F.;Thallman, R. M.;Smith, T. P. L.
继续验证重要经济性状的遗传标记，对于建立标记辅助选择作为养牛业的一种工具至关重要。本研究的目的是评估骨桥蛋白基因(SPP1)中的SNP(T-9/T-10)与跨越多个世代的代表12个创建品种的等位基因的大牛群体生长速度的关系。这个种群自1981年以来一直由美国肉类动物研究中心维持，并接受双胞胎比率的选择。该群体的表型记录包括双胞胎比率和排卵率，这为研究SPP1基因对生殖性状的潜在影响提供了机会。对2,701只动物进行了SPPL基因T-9/T-10多态的基因分型。基因分型数据，包括先前在6号染色体上分型的标记(BTA6)，结合系谱信息被用来估计所有14,714只有表型记录的动物的基因分型概率。女性的遗传概率被用来计算加性效应、显性效应和印记效应回归的自变量。在混合模型分析中，每个性状都在一个双性状模型中进行分析，其中单胎和双胎被视为单独的性状，基因回归适合作为固定效应。SPP1标记与初生重(P<；0.006)、断奶重(P<；0.007)和周岁重(P<；0.003)的关系与先前报道的SPPI基因对周岁重的影响是一致的。我们的数据支持这样的结论，即SNP成功地跟踪了影响牛生长的功能等位基因。先前未检测到的SNP对初生重和断奶重的影响表明，这个特殊的SPP1标记可以解释BTA6上的出生QTL和Hcw解释的部分表型差异。
Continued validation of genetic markers for economically important traits is crucial to establishing marker-assisted selection as a tool in the cattle industry. The objective of the current study was to evaluate the association of a SNP (T-9/T-10) in the osteopontin gene (SPP1) with growth rate in a large cattle population spanning multiple generations and representing alleles from 12 founding breeds. This population has been maintained at the US Meat Animal Research Center since 1981 and subjected to selection for twinning rate. Phenotypic records for this population included twinning rate and ovulation rate, providing an opportunity to examine the potential effects of SPP1 genotype on reproductive traits. A set of 2,701 animals was genotyped for the T-9/T-10 polymorphism at SPPL The genotypic data, including previously genotyped markers on chromosome 6 (BTA6), were used in conjunction with pedigree information to estimate genotypic probabilities for all 14,714 animals with phenotypic records. The genotypic probabilities for females were used to calculate independent variables for regressions of additive, dominance, and imprinting effects. Genotypic regressions were fit as fixed effects in a mixed model analysis, in which each trait was analyzed in a 2-trait model where single births were treated as a separate trait from twin births. The association of the SPP1 marker with birth weight (P < 0.006), weaning weight (P < 0.007), and yearling weight (P < 0.003) was consistent with the previously reported effects of SPPI genotype on yearling weight. Our data supports the conclusion that the SNP successfully tracks functional alleles affecting growth in cattle. The previously undetected effect of the SNP on birth and weaning weight suggests this particular SPP1 marker may explain a portion of the phenotypic variance explained by QTL for birth and HCW on BTA6.