Trehalose accumulation in Saccharomyces cerevisiae cells:: experimental data and structured modeling
Trehalose accumulation in Saccharomyces cerevisiae cells:: experimental data and structured modeling
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DOI：
10.1016/s1369-703x(03)00148-7
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发表时间：
2004-02-01
影响因子：
3.9
通讯作者：
Taillandier, P
中科院分区：
文献类型：
--
作者：
Aranda, JS;Salgado, E;Taillandier, P
几项研究表明，储备二糖海藻糖也保护环境压力下的酵母细胞。此外，人们认识到海藻糖可以提高酵母的活力，因此它的合成在面包酵母工业以及葡萄酒和啤酒生产中至关重要。在本研究中，我们采用补料分批培养的方法，对酿酒酵母进行碳源和氮源饥饿处理，以诱导海藻糖的生物合成和积累。我们的实验结果表明，酵母可以积累海藻糖高达13%的生物量干重（0.13克（海藻糖）克（生物量）（-1））在碳或氮饥饿。提出了一个分区和生化结构模型，以数学描述这些实验结果。模型的预测进行了比较与文献中的生长条件下获得的结果不同，在我们的实验中建立的，导致在一个非常好的协议，从而表明，所提出的假设基本上是正确的。(C)2003 Elsevier B. V.保留所有权利。
Several studies have shown that the reserve disaccharide trebalose also protects yeast cells under environmental stress. Besides, it is acknowledged that trehalose improves yeast viability so its synthesis has become paramount in baker's yeast industry, as well as in wine and beer production. In this work, starving carbon or nitrogen conditions were imposed to Saccharomyces cerevisiae growing in fed-batch cultivations in order to induce biosynthesis and accumulation of trehalose. Our experimental results show that yeast can accumulate trehalose up to 13% of biomass dry weight (0.13 g(trehalose) g(biomass)(-1)) under carbon or nitrogen starvation. A compartmentalized and biochemically structured model is proposed in order to mathematically describe these experimental findings. The model predictions were compared with literature results obtained under growth conditions different from those established in our experiments, resulting in a very good agreement and thus indicating that the proposed hypotheses are essentially correct. (C) 2003 Elsevier B.V. All rights reserved.