Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings
Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings
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DOI：
10.1093/oxfordjournals.jbchem.a021949
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发表时间：
1998-03-01
影响因子：
2.7
通讯作者：
Tzen, JTC
中科院分区：
文献类型：
--
作者：
Wu, LSH;Wang, LD;Tzen, JTC
油质蛋白是一种广泛存在于植物种子油体中的疏水蛋白质。水稻油体中存在分子量为18和16 kDa的两种不同的油质蛋白异构体，这两种异构体在水稻胚和糊粉层中的比例相似。为了研究与油质蛋白基因激活有关的DNA序列，对水稻18 kDa油质蛋白基因组克隆进行了测序，并将其5 '侧翼区与已知的水稻16 kDa油质蛋白基因进行了比较。两种水稻油质蛋白异构体的相应mRNA在授粉后7天出现，在成熟种子中消失，三酰甘油和油质蛋白在成熟的水稻芦苇中仅雅阁着油体的活跃组装而积累，并在萌发后的幼苗中部分被动员，约60%的水稻储存的三酰甘油没有被利用：胚中的油体在吸胀后5天内大部分被动员，而糊粉层中的油体在萌发后的幼苗中保持完整。
Oleosins are hydrophobic proteins localized abundantly in the oil bodies of plant seeds. Two distinct oleosin isoforms of molecular masses 18 and 16 kDa are present in rice oil bodies, These isoforms were found in similar ratio in rice embryos and aleurone layers, To survey potential DNA sequences involved in the activation of oleosin genes, a genomic clone of rice 18 kDa oleosin was sequenced, and its 5'-flanking region was compared with that of the known rice 16 kDa oleosin gene. Corresponding mRNAs of the two rice oleosin isoforms appeared seven days after pollination and vanished in mature seeds, Triacylglycerols and oleosins mere accumulated concomitantly in maturing rice reeds in accord with the active assembly of oil bodies, and partly mobilized in postgerminative seedlings, Approximately 60% of the stored triacylglycerols in rice were not utilized: while the majority of oil bodies in embryos were mobilized in five days after imbibition, those in aleurone layers remained intact in postgerminative seedlings.