Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium
Chemokine and Fgf signalling act as opposing guidance cues in formation of the lateral line primordium
复制标题
DOI：
10.1242/dev.080275
复制
发表时间：
2012-06-15
期刊：
影响因子：
4.6
通讯作者：
Xu, Qiling
中科院分区：
文献类型：
--
作者：
Breau, Marie A.;Wilson, Duncan;Xu, Qiling
许多细胞群的定向迁移作为一个连贯的群体发生。斑马鱼的后侧线提供了一个可行的模型，它是由从头到尾迁移的粘性原基形成的，并间隔地沉积未来的神经丘。我们发现，在迁移开始之前，原基的紧凑状态没有完全建立，因为具有侧线身份的孤立细胞存在于主要原基的尾部。这些分离的细胞被保留在适当的位置，使它们与迁移的原基融合，因为它的进步，后来有助于领导区和终端神经瘤。我们发现，孤立的侧线细胞的位置由两个拮抗性的线索：FGF信号吸引他们对原基，抵消Sdf 1 α/Cxcr 4 b介导的尾吸引。这些研究结果揭示了一种新的趋化作用的FGF信号，其中它使合并的侧线原基从最初的模糊图案成一个紧凑的一组迁移细胞。
The directional migration of many cell populations occurs as a coherent group. An amenable model is provided by the posterior lateral line in zebrafish, which is formed by a cohesive primordium that migrates from head to tail and deposits future neuromasts at intervals. We found that prior to the onset of migration, the compact state of the primordium is not fully established, as isolated cells with lateral line identity are present caudal to the main primordium. These isolated cells are retained in position such that they fuse with the migrating primordium as it advances, and later contribute to the leading zone and terminal neuromasts. We found that the isolated lateral line cells are positioned by two antagonistic cues: Fgf signalling attracts them towards the primordium, which counteracts Sdf1 alpha/Cxcr4b-mediated caudal attraction. These findings reveal a novel chemotactic role for Fgf signalling in which it enables the coalescence of the lateral line primordium from an initial fuzzy pattern into a compact group of migrating cells.