AN ENZYME-RELEASE ASSAY FOR NATURAL CYTO-TOXICITY
AN ENZYME-RELEASE ASSAY FOR NATURAL CYTO-TOXICITY
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DOI：
10.1016/0022-1759(83)90438-6
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
CALLEWAERT, DM
中科院分区：
文献类型：
--
作者：
KORZENIEWSKI, C;CALLEWAERT, DM
描述了一种灵敏的酶释放法测定天然细胞毒性[人淋巴细胞]。乳酸脱氢酶（LDH）从裂解的靶细胞[人白血病K562]释放的量的动力学测定，当与适当的对照和计算方法结合使用时，确定了对自然细胞毒性的敏感和精确测量。在实验误差范围内，该方法测定的细胞毒性百分比或动力学参数值与平行51Cr释放测定法测定的值相同。靶细胞LDH的自发释放量明显低于51Cr的自发释放量。这种酶释放细胞毒性试验方便、廉价、精确，应该适用于其他细胞毒性反应的研究，包括抗体依赖性和T细胞介导的反应。
A sensitive enzyme-release assay for natural cytotoxicity [of human lymphocytes] is described. The kinetic determination of the amount of the enzyme lactate dehydrogenase (LDH) released from lysed target cells [human leukemia K562] was determined to provide a sensitive and precise measure of natural cytotoxicity when used in conjunction with appropriate controls and calculational methods. Values for the percentage of cytotoxicity or kinetic parameters determiend by this method were identical, within experimental error, to values determined in parallel 51Cr release assays. The spontaneous release of LDH from the target cells tested was considerably lower than the spontaneous release of 51Cr. This enzyme-release cytotoxicity assay is convenient, inexpensive and precise, and should be applicable to the study of other cytotoxicity reactions, including antibody-dependent and T cell-mediated reactions.