Double in situ hybridization techniques in zebrafish
Double in situ hybridization techniques in zebrafish
复制标题
DOI：
10.1006/meth.2000.1147
复制
发表时间：
2001-04-01
期刊：
影响因子：
4.8
通讯作者：
Jowett, T
中科院分区：
文献类型：
--
作者：
Jowett, T
近年来，许多在斑马鱼胚胎中表达的基因已被克隆，并且在其分析中的第一步是通过原位杂交确定其转录本的空间和时间表达模式。通常有必要将表达模式与在同一发育时期表达的其他基因的表达模式联系起来。来自不同基因的转录物可以在互补或重叠结构域中表达。不同基因的表达模式可以通过在连续的组织切片上进行原位杂交来关联，但也可以用两种探针进行杂交并在同一组织中分别观察信号。有时也可以将原位杂交与免疫细胞化学结合联合收割机来定位组织特异性抗原。本文描述了为在斑马鱼中进行这些类型的分析而开发的方法。(C)北京：科学出版社.
Over recent years many genes expressed in zebrafish embryos have been cloned and a first step in their analysis is the determination of the spatial and temporal expression patterns of their transcripts by in situ hybridization. It is often necessary to relate the expression pattern to that of other genes expressed at the same period of development. Transcripts from different genes may be expressed in complementary or overlapping domains. The pattern of expression of different genes can be related by performing in situ hybridization on consecutive sections of tissue but it is also possible to perform hybridizations with two probes and to visualize the signals separately in the same tissue. Occasionally it is also possible to combine in situ hybridization with immunocytochemistry to localize tissue-specific antigens. Methods developed for performing these types of analyses in zebrafish are described in this article. (C) 2001 Academic Press.