Host range factor 1 from Lymantria dispar nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB-Ld652Y cells derived from L-dispar
Host range factor 1 from Lymantria dispar nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB-Ld652Y cells derived from L-dispar
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DOI：
10.1128/jvi.78.22.12703-12708.2004
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发表时间：
2004-11-01
影响因子：
5.4
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
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作者：
Ishikawa, H;Ikeda, M;Kobayashi, M
舞毒蛾核多角体病毒的宿主范围因子1（HRF-1）促进苜蓿银纹夜蛾核型多角体病毒（Autographa californica MNPV）在Ld 652 Y细胞中的复制。差异在这里，我们证明，限制美国白蛾核型多角体病毒复制在Ld 652 Y细胞是不是由于细胞凋亡，但可能是由于全球蛋白质合成阻滞，可以恢复由HRF-1。我们的数据还表明，HRF-1促进了其他两种杆状病毒，家蚕核型多角体病毒和甜菜夜蛾核型多角体病毒，其复制在Ld 652 Y细胞中是有限的复制病毒DNA没有成功的生产感染性子代病毒粒子的后代病毒粒子的生产。因此，HRF-1是Ld 652 Y细胞中NPV的生产性感染所需的基本病毒因子。
Host range factor 1 (HRF-1) of Lymantria dispar multinucleocapsid nucleopolyhedrovirus promotes Autographa californica MNPV replication in nonpermissive Ld652Y cells derived from L. dispar. Here we demonstrate that restricted Hyphantria cunea NPV replication in Ld652Y cells was not due to apoptosis but was likely due to global protein synthesis arrest that could be restored by HRF-1. Our data also showed that HRF-1 promoted the production of progeny virions for two other baculoviruses, Bombyx mori NPV and Spodoptera exigua MNPV, whose replication in Ld652Y cells is limited to replication of viral DNA without successful production of infectious progeny virions. Thus, HRF-1 is an essential viral factor required for productive infection of NPVs in Ld652Y cells.