Vanadium oxide thermal microsensor integrated in a microfluidic chip for detecting cholesterol and glucose concentrations
Vanadium oxide thermal microsensor integrated in a microfluidic chip for detecting cholesterol and glucose concentrations
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DOI：
10.1007/s00542-016-3090-1
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发表时间：
2017-07
期刊：
影响因子：
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通讯作者：
N. Inomata;Libao Pan;Zhuqing Wang;M. Kimura;T. Ono
中科院分区：
文献类型：
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作者：
N. Inomata;Libao Pan;Zhuqing Wang;M. Kimura;T. Ono
研制了一种基于氧化钒（VOx）微热敏电阻的高灵敏度热测量装置，用于检测基于酶反应的生物分子。VOx热敏电阻的测量原理依赖于其电阻的温度依赖性，这是由于酶反应产生的热量。该装置包括一个微流通道和腔室，以及在悬浮的Si 3 N4膜上用于热绝缘的VOxtrustors。将酶固定在传感器上的硅胶中。制作的VOx热敏电阻的电阻温度系数为− 1.3%/K。制作的器件具有0.30 mK/kHz的温度分辨率和4.9 mV/K的温度响应。胆固醇和葡萄糖的热测量证明使用酶促反应引起的热产生。葡萄糖和胆固醇的可检测浓度分别为30和15 µM/kHz。
A highly sensitive thermal measurement device based on a vanadium oxide (VOx) microthermistor is developed for detecting biological molecules based on enzymatic reactions. The measurement principle of the VOxthermistor relies on the temperature dependence of its electrical resistance owing to the heat generation of enzymatic reactions. The device comprises a microfluidic channel and chambers, and VOxthermistors on a suspended Si3N4membrane for thermal insulation. The enzyme is immobilized in silica gel on the sensor. The temperature coefficient of the resistance of the fabricated VOxthermistor is −1.3 %/K. The fabricated device has a temperature resolution of 0.30 mK/√Hz and a temperature response of 4.9 mV/K. Thermal measurements of cholesterol and glucose are demonstrated using the heat production caused by enzymatic reactions. The detectable concentrations of glucose and cholesterol were 30 and 15 µM/√Hz, respectively.