SWINE DUST CAUSES INTENSE AIRWAYS INFLAMMATION IN HEALTHY-SUBJECTS
SWINE DUST CAUSES INTENSE AIRWAYS INFLAMMATION IN HEALTHY-SUBJECTS
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DOI：
10.1164/ajrccm.150.4.7921472
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发表时间：
1994-10-01
影响因子：
24.7
通讯作者：
MALMBERG, PO
中科院分区：
文献类型：
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作者：
LARSSON, KA;EKLUND, AG;MALMBERG, PO
14名健康的非吸烟受试者暴露于猪尘，而称重猪3至5小时，在猪禁闭建设。艺术，但一名参与者是以前未暴露于猪尘。在暴露前后进行支气管肺泡灌洗（BAL）并抽取血液样本。通过在呼吸区携带的过滤盒中进行空气采样来测量总粉尘和内毒素。空气中内毒素浓度为0.6（范围：0.08 - 1.3）μ g/m3，总粉尘浓度为13.5（范围：5.6 - 24.0）mg/m3。空气中氨、二氧化碳和硫化氢的浓度较低。暴露导致三名受试者发烧，六名受试者感到不适和困倦。与暴露前值相比，在暴露后1天（类似于22小时）获得的BAL液中观察到中性粒细胞增加75倍，单核细胞增加两到三倍，嗜酸性粒细胞显着增加（p <0.01）所有细胞类型）。BAL液中的纤维连接蛋白和白蛋白浓度显著增加（两者均为p <0.01）。暴露后6 h外周血白细胞数几乎增加一倍，1 d后仍显著升高。暴露后第1天血清类粘蛋白和C反应蛋白（CRP）浓度显著升高。我们得出结论，暴露于猪圈建筑物中的猪尘诱导了气道中的强烈炎症反应，通过BAL评估。气道细胞反应的特征在于中性粒细胞的急剧增加。猪粉尘中引起反应的成分尚不清楚。
Fourteen healthy nonsmoking subjects were exposed to swine dust while weighing pigs for 3 to 5 h in a swine-confinement building. Art but one participant was previously unexposed to swine dust. Bronchoalveolar lavage (BAL) was performed and blood samples were drawn before and after exposure. Total dust and endotoxins were measured by air sampling in filter cassettes carried in the breathing zone. The air concentration of endotoxins was 0.6 (range, 0.08 to 1.3) mu g/m(3), and of total dust was 13.5 (range, 5.6 to 24.0) mg/m(3). The air concentrations of ammonia, carbon dioxide, and hydrogen sulfide were low. The exposure induced fever in three and malaise and drowsiness in six of the subjects. Compared with preexposure values, a 75-fold increase in neutrophilic granulocytes, a two- to threefold increase in mononuclear cells, and a significant increase in eosinophilic granulocytes were observed in the BAL fluid obtained 1 day (similar to 22 h) after exposure (p < 0.01 for all cell types). The fibronectin and albumin concentrations in the BAL fluid significantly increased (p < 0.01 for both). The number of leukocytes in peripheral blood was almost doubled 6 h after exposure and was still significantly elevated after 1 day. Blood concentrations of orosomucoid and C-reactive protein (CRP) were significantly increased 1 day after exposure. We conclude that exposure to swine dust in a swine-confinement building induces an intense inflammatory reaction in the airways as assessed by BAL. The airway cellular response is characterized by a dramatic increase in neutrophils. The components in the swine dust that cause the reaction are not clear.