Moment Analysis Theory for Kinetic Study of Intermolecular Interaction by Affinity Capillary Electrophoresis
Moment Analysis Theory for Kinetic Study of Intermolecular Interaction by Affinity Capillary Electrophoresis
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DOI：
10.1246/bcsj.20160068
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发表时间：
2016-04
影响因子：
4
通讯作者：
K. Miyabe;Nozomu Suzuki
中科院分区：
文献类型：
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作者：
K. Miyabe;Nozomu Suzuki
将矩分析(MA)理论引入到亲和毛细管电泳法(ACE)洗脱峰分析中。建立了新的力矩方程，这些方程对于从ACE数据解析确定分子间相互作用的缔合(Ka)和解离(Kd)速率常数是必不可少的。MA理论与ACE程序的结合形成了一种研究分子间相互作用动力学的方法(MA-ACE)。由于ACE经常被用来确定分子间相互作用的结合(缔合平衡)常数，因此从ACE洗脱峰也能确定ka和kd一定很重要。尝试用MA法分析一篇已发表的论文中的ACE洗脱峰，以确定速率常数。分析测定了三种硼酸与果糖分子间相互作用的ka和kd。与另一种基于宏观的方法测定的结果具有可比性。
The moment analysis (MA) theory was introduced into the analysis of elution peak profiles measured by affinity capillary electrophoresis (ACE). New moment equations were developed, which were essential for analytically determining the association (ka) and dissociation (kd) rate constants of intermolecular interactions from the ACE data. The combination of the MA theory and ACE procedure leads to a method for the kinetic study of intermolecular interactions (MA-ACE). Because ACE has quite frequently been used for determining binding (association equilibrium) constants of intermolecular interactions, it must be important that ka and kd can also be determined from ACE elution peaks. An attempt was made to analyze ACE elution peaks in a published paper by the MA method to determine the rate constants. The values of ka and kd were analytically determined for the intermolecular interaction between three boronic acids and fructose. They were comparable with those determined by the other method based on the macro...