Expression of the alpha beta T-cell receptor is necessary for the generation of the thymic medulla.
Expression of the alpha beta T-cell receptor is necessary for the generation of the thymic medulla.
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DOI：
10.1155/1993/56290
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Owen MJ
中科院分区：
文献类型：
--
作者：
Palmer DB;Viney JL;Ritter MA;Hayday AC;Owen MJ
用免疫组织学方法观察了先天性αβ T细胞受体（TCRαβ）表达缺失小鼠胸腺的结构。在这些小鼠中，通过同源重组将确定的突变引入TCR c基因。通过使用皮质或髓质上皮和主要组织相容性复合物抗原的特异性抗体，这些小鼠的皮质上皮网络与正常小鼠相比基本上没有改变。与此相反，胸腺髓质的大小大大减少。该分析表明，αβ TCR而非γδ TCR的表达对于建立胸腺髓质是必需的，并表明髓质上皮细胞的生长可能需要与TCRαβ表达细胞接触。
The architecture of the thymus of mice that congenitally fail to express the αβ T-cell receptor (TCRαβ) has been examined by immunohistology. In these mice, a defined mutation was introduced into the TCRc gene by homologous recombination. By using antibodies specific for cortical or medullary epithelium and for major histocompatibility complex antigens, the network of cortical epithelium in these mice was shown to be essentially unaltered in comparison with that of normal mice. In contrast, the thymic medulla was considerably reduced in size. This analysis shows that expression of the αβ TCR but not the γδ TCR is obligatory for establishing the thymic medulla and suggests that the growth of medullary epithelial cells may require contact with TCRαβ-expressing cells.