Lonicera caerulea L. Polyphenols Alleviate Oxidative Stress-Induced Intestinal Environment Imbalance and Lipopolysaccharide-Induced Liver Injury in HFD-Fed Rats by Regulating the Nrf2/HO-1/NQO1 and MAPK Pathways
Lonicera caerulea L. Polyphenols Alleviate Oxidative Stress-Induced Intestinal Environment Imbalance and Lipopolysaccharide-Induced Liver Injury in HFD-Fed Rats by Regulating the Nrf2/HO-1/NQO1 and MAPK Pathways
复制标题
蓝靛果多酚通过调节 Nrf2/HO-1/NQO1 和 MAPK 通路缓解 HFD 喂养大鼠氧化应激引起的肠道环境失衡和脂多糖引起的肝损伤
DOI：
10.1002/mnfr.201901315
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发表时间：
2020-05-04
影响因子：
5.2
通讯作者：
Meng, Xianjun
中科院分区：
文献类型：
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作者：
Li, Bin;Cheng, Zhen;Meng, Xianjun
范围 本研究探讨了蓝靛果多酚 (LCP) 在氧化应激损伤 (OSD) 大鼠模型中通过核因子红细胞 2 相关因子 2/血红素加氧酶 -1 (HO-1)/NQO1 和丝裂原激活蛋白激酶 (MAPK) 通路对肠道环境和脂多糖 (LPS) 诱导的肝损伤的调节作用。方法和结果LCP 的研究使用 Sprague Dawley 大鼠建立了高脂饮食诱导的 OSD 模型。在结肠中，用 LCP 治疗 8 周可改善肠道通透性增强（胰高血糖素样肽 2 含量和 Occludin 蛋白增加，而 Claudin-2 蛋白减少）、肠道炎症（促炎细胞因子水平，如肿瘤坏死因子-α、白细胞介素 6、环氧合酶 2 和核因子 kappa-B p65 (NF-kappa B p65) 水平降低）和肠道 OSD（通过调节Nrf2/HO-1/NQO1 途径）。此外，LCP 通过抑制 NF-kappa B p65 的核转位和 MAPK 信号通路的激活来减轻 LPS 诱导的肝损伤。此外，芽孢杆菌、乳杆菌目、乳杆菌科、乳杆菌、Akkermansia、放线菌、变形菌、Rothia 和 Blautia 被发现是与 OSD 和 LPS 诱导的大鼠肠道肝损伤相关的关键肠道微生物分类群。结论 LCP 治疗可能通过抑制氧化应激相关途径和改变肠道微生物群的组成来调节肠道环境并减轻肝损伤。
Scope This study investigates the modulatory effects of Lonicera caerulea L. polyphenols (LCPs) on the intestinal environment and lipopolysaccharide (LPS)-induced liver injury via the nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (HO-1)/NQO1 and mitogen-activated protein kinase (MAPK) pathways in a rat model of oxidative stress damage (OSD).Methods and results To examine the prebiotic properties of LCP, a model of high-fat-diet-induced OSD is established using Sprague Dawley rats. In the colon, treatment with LCP for 8 weeks ameliorates enhanced intestinal permeability (glucagon-like peptide-2 content and occludin protein increase, whereas claudin-2 protein decreases), intestinal inflammation (levels of pro-inflammatory cytokines, such as tumor necrosis factor-alpha, interleukin-6, cyclooxygenase-2, and nuclear factor kappa-B p65 (NF-kappa B p65), decrease), and intestinal OSD (through regulation of the Nrf2/HO-1/NQO1 pathway). Moreover, LCP alleviates LPS-induced liver injury by suppressing the nuclear translocation of NF-kappa B p65 and activation of the MAPK signaling pathway. Additionally, Bacilli, Lactobacillales, Lactobacillaceae, Lactobacillus, Akkermansia, Actinobacteria, Proteobacteria, Rothia, and Blautia are found to be the key intestinal microbial taxa related to intestinal OSD and LPS-induced liver injury in rats.Conclusion LCP treatment potentially modulates the intestinal environment and alleviates liver injury by suppressing oxidative-stress-related pathways and altering the composition of the intestinal microbiota.