Isolation and Functional Analysis of the Poplar RbcS Gene Promoter
Isolation and Functional Analysis of the Poplar RbcS Gene Promoter
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DOI：
10.1007/s11105-012-0482-y
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发表时间：
2013-02
影响因子：
2.1
通讯作者：
Like Wang;Lisha Li;Lina Xu;Jie Zhou;Huihui Zhuang;Xidong Gong;Mingxiu Wang;S. S. Sun-S.;Q. Zhuge
中科院分区：
文献类型：
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作者：
Like Wang;Lisha Li;Lina Xu;Jie Zhou;Huihui Zhuang;Xidong Gong;Mingxiu Wang;S. S. Sun-S.;Q. Zhuge
根据AtRBCS-2B cDNA序列与杨树基因组序列的同源性分析，从杨树中分离并克隆了一个组织特异性启动子Pt-RbcS。Pt-RbcS基因序列分析表明，该基因启动子区存在与光反应有关的顺式调控元件，包括ATCT基序、BoxI、GAG基序、I-box、G-box、BoxII、GATA基序和TCT基序。在转β-葡萄糖醛酸酶（GUS）基因烟草中，GUS基因在叶片和茎中表达，而在根中不表达。转基因白杨株系的构建体携带GUS基因的truncatedPt-RbcS启动子驱动显示不同的表达模式为五个不同的启动子结构。Pt-RbcS启动子在叶片和茎等光合组织中优先表达。此外，对1，547 bp Pt-RbcS启动子区的缺失分析表明，927 bp的DNA片段是Pt-RbcS在绿色组织中表达的关键。因此，我们的研究表明，来自杨属植物的Pt-RbcS启动子可以用于遗传改良木本植物的光合效率。
A tissue-specific promoter,Pt-RbcS, fromPopuluswas isolated and cloned based on alignment ofAtRBCS-2B cDNA with genomicPopulussequences. Sequence analysis ofPt-RbcSrevealed cis-acting regulatory elements in the promoter region, including an ATCT-motif, BoxI, GAG-motif, I-box, G-box, BoxII, GATA-motif, and TCT-motif, which are involved in light responses. In transgenic tobacco lines carrying theβ-glucuronidase (GUS) gene driven by thePt-RbcSpromoter, GUS expression was detected in leaves and stems, but not in roots. Transgenic poplar lines harboring constructs carrying the GUS gene driven by truncatedPt-RbcSpromoters revealed distinctive expression patterns for five different promoter constructs. ThePt-RbcSpromoter was expressed preferentially in photosynthetic tissues such as leaves and stems. Moreover, deletion analysis of the 1,547 bpPt-RbcSpromoter region revealed that a 927-bp DNA segment is critical for expression ofPt-RbcSin green tissues. Overall, our study suggests that thePt-RbcSpromoter fromPopuluscould be applied to genetically improve the photosynthetic efficiency of woody plants.