A decade of discovery: CRISPR functions and applications
A decade of discovery: CRISPR functions and applications
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DOI：
10.1038/nmicrobiol.2017.92
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发表时间：
2017-07-01
影响因子：
28.3
通讯作者：
Horvath, Philippe
中科院分区：
文献类型：
--
作者：
Barrangou, Rodolphe;Horvath, Philippe
今年是鉴定出CRISPR-Cas作为细菌适应性免疫系统的生物学功能的十周年。在短短十年里，CRISPR-Cas系统的表征已经在细菌和古细菌中建立了一种新的适应性免疫手段，并加深了我们对原核生物与其环境之间相互作用的理解，基于crispr的分子机器已经被重新用于实现基因组编辑革命。在这里，我们回顾了为发现CRISPR及其功能铺平道路的历史里程碑，并讨论了已经出现的相关技术应用，重点是微生物学。最后，我们对该领域对科学及其他领域的影响提供了一个视角。
This year marks the tenth anniversary of the identification of the biological function of CRISPR-Cas as adaptive immune systems in bacteria. In just a decade, the characterization of CRISPR-Cas systems has established a novel means of adaptive immunity in bacteria and archaea and deepened our understanding of the interplay between prokaryotes and their environment, and CRISPR-based molecular machines have been repurposed to enable a genome editing revolution. Here, we look back on the historical milestones that have paved the way for the discovery of CRISPR and its function, and discuss the related technological applications that have emerged, with a focus on microbiology. Lastly, we provide a perspective on the impacts the field has had on science and beyond.