Serotonin release into plasma during common carotid artery thrombosis in rats.
Serotonin release into plasma during common carotid artery thrombosis in rats.
复制标题
大鼠颈总动脉血栓形成期间血清素释放到血浆中。
DOI：
10.1161/01.str.23.6.870
复制
发表时间：
1992
期刊：
影响因子：
8.3
通讯作者：
Globus,MY
中科院分区：
文献类型：
--
作者：
Wester,P;Dietrich,WD;Prado,R;Watson,BD;Globus,MY
我们已经验证了血小板衍生的血清素在脑血管血栓形成过程中释放到血流中的假设。使用光敏染料孟加拉红和氩泵染料激光照射，在 22 只麻醉的成年雄性 Wistar 大鼠中通过光化学方法产生非闭塞性颈总动脉血栓。在光血栓形成之前、期间和之后，通过动脉内体内微透析将探针放置在血栓形成部位远端，记录光血栓形成之前、期间和之后的血浆血清素水平。在颈总动脉血栓形成期间和之后，血清素水平显着增加至峰值 781 nmol/l（方差分析 p 小于 0.001），与基线水平相比增加了 15 倍。增加的血清素水平逐渐下降，但在 90 分钟内仍显着升高。颈动脉血栓的超微结构分析发现，在脱颗粒的各个阶段都有大量聚集的血小板。这些结果首次直接证明，在颈总动脉血栓形成的急性期期间和之后，血浆中发生了血清素积累。血浆血清素水平升高可能在先前在大血管血栓性中风模型中记录的脑血流量和血脑屏障异常中发挥重要作用。
We have tested the hypothesis that platelet-derived serotonin is released into the bloodstream during cerebrovascular thrombosis.Nonocclusive common carotid artery thrombosis was produced photochemically in 22 anesthetized adult male Wistar rats using the photosensitizing dye rose bengal and irradiation with an argon-pumped dye laser. Plasma serotonin levels were recorded before, during, and after photothrombosis by intra-arterial in vivo microdialysis with the probe placed distal to the site of thrombosis.During and immediately after the common carotid artery thrombosis, serotonin levels increased significantly to a peak value of 781 nmol/l (p less than 0.001 by analysis of variance), representing a 15-fold increase compared with baseline levels. The increased serotonin levels gradually decreased but remained significantly elevated for 90 minutes. Ultrastructural analysis of the carotid thrombi identified a dense mass of aggregated platelets at various stages of degranulation.These results are the first to demonstrate directly that serotonin accumulation occurs in plasma during and after the acute phase of common carotid artery thrombosis. Increased plasma serotonin levels may play a major role in the cerebral blood flow and blood-brain barrier abnormalities previously documented in this model of large-vessel thrombotic stroke.