Two quenching groups are better than one: A robust strategy for constructing HOCl fluorescent probe with minimized background fluorescence and ultra-high sensitivity and its application of HOCl imaging in living cells and tissues
Two quenching groups are better than one: A robust strategy for constructing HOCl fluorescent probe with minimized background fluorescence and ultra-high sensitivity and its application of HOCl imaging in living cells and tissues
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两个猝灭基团优于一个猝灭基团：一种构建具有最小背景荧光和超高灵敏度的HOCl荧光探针的稳健策略及其在活细胞和组织中HOCl成像的应用
DOI：
10.1016/j.snb.2020.127890
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Hongyan Sun
中科院分区：
文献类型：
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作者：
Guansheng Zheng;Zejun Li;Qinya Duan;Ke Cheng;Yong He;Shumei Huang;Huatang Zhang;Yin Jiang;Yongguang Jia;Hongyan Sun
我们在此报告的荧光探针配备有双淬灭基团表现出上级的灵敏度比探针与单淬灭基团。重要的是，通过这种策略，具有双淬灭基团的探针通过两种不同的反应机制与HOCl反应，这减少了与其他竞争分析物的可能的副反应并增强了探针的选择性。作为概念验证研究，我们设计并合成了具有双猝灭基团DQ-HOCl的探针来检测HOCl，这是最重要的ROS之一，与许多疾病相关。此外，为了比较，还合成了两个具有单淬灭基团的对照探针MQ-HOCl-1和MQ-HOCl-2。荧光分析表明，与单淬灭基团的探针相比，DQ-HOCl确实显示出超高的灵敏度和选择性。此外，该探针已成功地用于活细胞中的外源性/内源性HOCl成像。此外，由于双光子探针的性质增加了穿透深度和降低了组织自发荧光，因此将DQ-HOCl用于显示大鼠肾组织中的HOCl。
We report herein fluorescent probes equipped with dual-quenching groups exhibiting superior sensitivity than probes with mono-quenching groups. Importantly, with this strategy, the probe with dual-quenching groups react with HOCl through two distinct reaction mechanisms, which reduce the plausible side reactions with other competing analytes and enhance the probe’s selectivity. As a proof-of-concept study, we designed and synthesized a probe with dual-quenching groupsDQ-HOClto detect HOCl, which is one of the most important ROS and linked with a number of diseases. In addition, two control probes with mono-quenching groups,MQ-HOCl-1andMQ-HOCl-2, were also synthesized for comparison purpose. Fluorescent assays demonstrated thatDQ-HOClindeed shows ultra-high sensitivity and selectivity compared with probes with mono-quenching groups. Furthermore, the probe has been successfully utilized to imaging exogenous/endogenous HOCl in living cells. Moreover,DQ-HOClwas applied to visualize HOCl in kidney tissues from rat due to the increased penetration depth and lower tissue autofluorescence from the nature of two-photon probes.