Stochastic spineless expression creates the retinal mosaic for colour vision
Stochastic spineless expression creates the retinal mosaic for colour vision
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DOI：
10.1038/nature04615
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发表时间：
2006-03-09
期刊：
影响因子：
64.8
通讯作者：
Desplan, C
中科院分区：
文献类型：
--
作者：
Wernet, MF;Mazzoni, EO;Desplan, C
果蝇的色觉是由存在于每个小眼中的R7和R8感光细胞实现的。果蝇视网膜包含两种类型的小眼，称为“苍白”和“黄色”，由R7和R8细胞中表达的不同视紫红质对定义。与人类视锥光感受器相似，这些小眼亚型随机分布在视网膜中。在R7细胞中选择淡黄色小眼，然后将它们的命运强加给R8。在这里，我们报告说，果蝇二恶英受体无刺是必要的，也是足够的小眼马赛克的形成。在R7细胞的大亚群中，在化蛹中期的无刺表达的短爆发先于视紫红质表达。在无刺突变体中，所有的R7和大多数R8细胞采用苍白的命运，而无刺的过度表达足以诱导黄色R7的命运。因此，这项研究表明，色觉所需的整个视网膜马赛克是由一个单一的转录因子，无脊椎的随机表达。
Drosophila colour vision is achieved by R7 and R8 photoreceptor cells present in every ommatidium. The fly retina contains two types of ommatidia, called 'pale' and 'yellow', defined by different rhodopsin pairs expressed in R7 and R8 cells. Similar to the human cone photoreceptors, these ommatidial subtypes are distributed stochastically in the retina. The choice between pale versus yellow ommatidia is made in R7 cells, which then impose their fate onto R8. Here we report that the Drosophila dioxin receptor Spineless is both necessary and sufficient for the formation of the ommatidial mosaic. A short burst of spineless expression at mid-pupation in a large subset of R7 cells precedes rhodopsin expression. In spineless mutants, all R7 and most R8 cells adopt the pale fate, whereas overexpression of spineless is sufficient to induce the yellow R7 fate. Therefore, this study suggests that the entire retinal mosaic required for colour vision is defined by the stochastic expression of a single transcription factor, Spineless.