RacA, a bacterial protein that anchors chromosomes to the cell poles
RacA, a bacterial protein that anchors chromosomes to the cell poles
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DOI：
10.1126/science.1079914
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发表时间：
2003-01-24
期刊：
影响因子：
56.9
通讯作者：
Losick, R
中科院分区：
文献类型：
--
作者：
Ben-Yehuda, S;Rudner, DZ;Losick, R
真核细胞染色体在有丝分裂过程中锚定在纺锤体上，但在细菌染色体分离过程中没有这种结构。当姐妹染色体在枯草芽孢杆菌孢子形成过程中分离时，复制起点区域迁移到细胞的两极。起源区域是否以及如何固定在极点尚未确定。在这里，我们描述了一种发育蛋白，RacA，作为起源区域和细胞两极之间的桥梁。我们认为RacA在靠近原点的一个类似着丝粒的元件上组装成一个粘性补丁，导致染色体粘在两极。
Eukaryotic chromosomes are anchored to a spindle apparatus during mitosis, but no such structure is known during chromosome segregation in bacteria. When sister chromosomes are segregated during sporulation in Bacillus subtilis, the replication origin regions migrate to opposite poles of the cell. If and how origin regions are fastened at the poles has not been determined. Here we describe a developmental protein, RacA, that acts as a bridge between the origin region and the cell poles. We propose that RacA assembles into an adhesive patch at a centromere-like element near the origin, causing chromosomes to stick at the poles.