Comparative effects between electronic and cigarette smoke in human keratinocytes and epithelial lung cells
Comparative effects between electronic and cigarette smoke in human keratinocytes and epithelial lung cells
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DOI：
10.1016/j.tiv.2014.04.012
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发表时间：
2014-08-01
影响因子：
3.2
通讯作者：
Valacchi, G.
中科院分区：
文献类型：
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作者：
Cervellati, F.;Muresan, X. M.;Valacchi, G.
关于电子烟有害影响的信息很少且不一致，因此，由于电子烟（e-CIG）的使用作为限制吸烟的工具越来越受欢迎，因此迫切需要确定商业e-CIG的毒性。皮肤（HaCaT）和肺（A549）细胞是香烟烟雾（CS）的主要目标，使用体外系统暴露于e-CIG蒸汽和CS。通过超微结构形态学、台盼蓝排斥试验和LDH测定分析了两种细胞类型中暴露的细胞毒性效应。此外，促炎细胞因子测定的Bio-CIG assay. e-CIG的细胞毒性成分被抑制的调味化合物，并在较小程度上，尼古丁，虽然他们的影响是有害的CS。单独的湿润剂没有表现出细胞毒性，但诱导细胞因子和促炎介质的释放。根据我们的结果，我们可以说，暴露于电子烟蒸汽的结果远远低于暴露于CS的毒性。事实上，除了香料和尼古丁的有害作用之外，即使是单独的湿润剂也能够引起细胞因子的释放。这项研究将有望促进更安全的电子香烟的发展，以帮助人们戒烟。(C)2014爱思唯尔有限公司版权所有。
Information about the harmful effects of vaping is sparse and inconsistent, therefore, since the use of electronic cigarettes (e-CIGs) has become increasingly popular as a tool to limit tobacco smoking, it is urgent to establish the toxicity of the commercial e-CIGs.Skin (HaCaT) and lung (A549) cells, the main targets of cigarette smoke (CS), were exposed to e-CIG vapor and CS using an in vitro system. The cytotoxic effect of the exposure was analyzed in both cell types by ultrastructural morphology, Trypan Blue exclusion test and LDH assay. In addition, pro-inflammatory cytokines were measured by the Bio-Plex assay.The cytotoxic components of e-CIG were restrained to the flavoring compound and, to a lesser extent, to nicotine although their effects were less harmful to that of CS. Humectants alone exhibited no cytotoxicity but induced the release of cytokines and pro-inflammatory mediators.Based on our results, we can state that exposure to e-CIG vapors results in far less toxic than exposure to CS. In fact, besides the deleterious effect of flavor and nicotine, even the humectants alone are able to evocate cytokines release. This study will hopefully promote the development of safer e-CIGs to help people quit smoking. (C) 2014 Elsevier Ltd. All rights reserved.