Cell cycle-dependent dynamics of cytoskeleton involving mitochondrial redistribution in hamster embryos.
Cell cycle-dependent dynamics of cytoskeleton involving mitochondrial redistribution in hamster embryos.
复制标题
细胞骨架的细胞周期依赖性动力学涉及仓鼠胚胎中线粒体的重新分布。
DOI：
10.1111/j.1439-0531.2012.02143.x
复制
发表时间：
2013
影响因子：
1.7
通讯作者：
Matsuzaki M and Suzuki H.
中科院分区：
文献类型：
--
作者：
Kabashima K;Yoshinaga D;Fang J;Matsuzaki M and Suzuki H.
内容研究了仓鼠胚胎细胞周期间期和M期线粒体与细胞骨架的相互作用。用nocodazole， cytochalasin D或两种抑制剂的组合培养两个细胞胚胎1小时，然后在10 000×gfor离心2分钟。对照胚仅离心，不加抑制剂处理。对离心后的胚胎进行荧光染色，观察活跃线粒体的分布和细胞核结构。在对照2细胞胚胎中，大部分线粒体聚集在核周区域，一些线粒体聚集在细胞皮层。两种抑制剂和离心均不影响卵裂球间期线粒体的分布。然而，在nocodazole和细胞松弛素D联合离心处理的间期卵裂球中，71% （n = 41）的线粒体向离心极方向旋转，这表明微管和微丝都可能参与细胞周期间期线粒体的再分配。相反，当使用包括细胞松弛素D在内的所有药物处理M期卵裂球时，线粒体通常在单极簇中脱位，这表明在细胞周期的M期，微丝而非微管可能参与线粒体的重新分配。这些数据表明，无论细胞周期处于什么阶段，微丝都在线粒体再分配中发挥作用，微管可能在间期与线粒体密切相关，但在M期与线粒体分离。
ContentsMitochondria–cytoskeleton interactions were studied in the hamster embryos during interphase and M phase of the cell cycle. Two‐cell embryos were cultured for 1 h with nocodazole, cytochalasin D or in a combination of both inhibitors and then centrifuged at 10 000×gfor 2 min. The control embryos were only centrifuged with no inhibitor treatment. Centrifuged embryos were fluorescently stained to examine the distribution of active mitochondria and nuclear configuration. In the control 2‐cell embryos, most mitochondria were accumulated at the perinuclear region with some at the cell cortex. Neither each inhibitor nor centrifugation did affect the distribution of mitochondria in interphase blastomeres. However, mitochondria were spun down towards the centrifugal pole in 71% (n = 41) of the interphase blastomeres treated with centrifugation following a combination of nocodazole plus cytochalasin D, suggesting that both microtubules and microfilaments may involve in mitochondrial redistribution during interphase of the cell cycle. In contrast, when M‐phase blastomeres were treated with all drug treatments applied, including cytochalasin D, mitochondria had been usually dislocated in a unipolar cluster, suggesting that microfilaments, not microtubules, may involve in the mitochondrial redistribution during M phase of the cell cycle. The data indicate that microfilaments function in mitochondrial redistribution regardless of the stages of the cell cycle and that microtubules may strongly associate with mitochondria during the interphase but dissociate from them during the M phase.