Pyruvate carboxylase: affinity labelling of the magnesium adenosine triphosphate binding site.
Pyruvate carboxylase: affinity labelling of the magnesium adenosine triphosphate binding site.
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丙酮酸羧化酶：三磷酸腺苷镁结合位点的亲和标记。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
D. Keech
中科院分区：
文献类型：
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作者：
S. Easterbrook;J. Wallace;D. Keech
ATP的2'，3'-二醛衍生物（oATP）通过高碘酸盐氧化制备，并且根据以下标准被认为是有效的亲和标记。该衍生物的镁络合物（Mg-oATP 2）在乙酰辅酶A依赖性和非依赖性酶活性中显示出相对于MgATP 2-的线性竞争性抑制剂，但相对于碳酸氢盐是非竞争性抑制剂，并且相对于丙酮酸是非竞争性抑制剂。用硼氢化钠还原Mg-oATP与丙酮酸羧化酶共价结合，同时使酶不可逆失活。
The 2' , 3'-dialdehyde derivative of ATP (oATP) was prepared by periodate oxidation and on the following criteria was considered to be an effective affinity label. The magnesium complex of this derivative (Mg-oATP2) was shown to ba linear competitive inhibitor with respect to MgATP2-in both the acetyl-CoA-dependent and -independent activities of the enzyme but was a non-competitive inhibitor with respect to bicarbonate, and an uncompetitive inhibitor with respect to pyruvate. Mg-oATP was covalently bound to pyruvate carboxylase by reduction using sodium borohydride with concurrent irreversible inactivation of the enzyme...