Involvement of PpWRKY70 in the methyl jasmonate primed disease resistance against Rhizopus stolonifer of peaches via activating phenylpropanoid pathway
Involvement of PpWRKY70 in the methyl jasmonate primed disease resistance against Rhizopus stolonifer of peaches via activating phenylpropanoid pathway
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PpWRKY70 通过激活苯丙素途径参与茉莉酸甲酯引发桃子根霉的抗病性
DOI：
10.1016/j.postharvbio.2021.111466
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发表时间：
2021-01-17
影响因子：
7
通讯作者：
Zheng, Yonghua
中科院分区：
文献类型：
--
作者：
Ji, Nana;Wang, Jing;Zheng, Yonghua
研究了茉莉酸甲酯（MeJA）诱导桃抗根霉的分子机制。MeJA处理提高了苯丙氨酸解氨酶（PAL）和4-香豆酸辅酶A连接酶（4CL）的活性，促进了总酚、总黄酮和木质素含量以及绿原酸、新绿原酸和表儿茶素等主要单体酚类化合物的积累。此外，MeJA处理的桃果实中PpPAL和Pp 4CL的转录水平在接种病原菌后显著快速增强，表明MeJA通过增强苯丙烷途径引发桃的启动防御。此外，MeJA引发了PpWRKY 70的表达，其被鉴定为通过结合到PpPAL和Pp 4CL的W盒的转录激活因子。两者合计，我们的结果表明，PpWRKY 70是通过激活苯丙酸途径参与MeJA引发的防御。
The molecular mechanisms underlying methyl jasmonate (MeJA) primed defense against Rhizopus stolonifer through regulating phenylpropanoid pathway in peaches and the involvement of WRKY transcription factor were investigated. MeJA treatment enhanced the activity of phenylalanine ammonia-lyase (PAL) and 4-coumarateCoA ligase (4CL), and promoted the accumulation of total phenolics, total flavonoids and lignin content as well as the main individual phenolic compounds, including chlorogenic acid, neochlorogenic acid and epicatechin. Moreover, transcripts of PpPAL and Pp4CL transcription were substantially and rapidly enhanced upon inoculation with the pathogen in MeJA-treated fruit, indicating that MeJA triggered a priming defense via enhancing phenylpropanoid pathway in peaches. Furthermore, MeJA primed the expression of PpWRKY70, which was identified as a transcription activator of PpPAL and Pp4CL via binding to their W-boxes. Taken together, our results demonstrate that PpWRKY70 is involved in the MeJA-primed defense by activating the phenylpropanoid pathway.