MAPPING OF ORGANIC-SOLVENT TOLERANCE GENE OSTA IN ESCHERICHIA-COLI K-12
MAPPING OF ORGANIC-SOLVENT TOLERANCE GENE OSTA IN ESCHERICHIA-COLI K-12
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DOI：
10.1271/bbb.58.1231
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发表时间：
1994-07-01
影响因子：
1.6
通讯作者：
HORIKOSHI, K
中科院分区：
文献类型：
--
作者：
AONO, R;NEGISHI, T;HORIKOSHI, K
大肠杆菌K - 12不同菌株对有机溶剂的耐受程度存在差异。对正己烷耐受菌株的遗传分析表明，许多基因与溶剂耐受表型有关。其中一个这样的基因，被命名为ostA，定位在1.2分钟处，靠近pdxA。将ostA从正己烷敏感菌株转导至正己烷耐受菌株产生了正己烷敏感的转导子。该敏感转导子通过从耐受菌株转导ostA恢复了对正己烷的耐受性。因此，ostA基因是决定大肠杆菌有机溶剂耐受水平的基因之一。
The extent of organic solvent tolerance was variable among strains of Escherichia coli K-12. Genetic analyses of n-hexane-tolerant strains indicated that a number of genes were involved in the solvent-tolerance phenotype. One such gene, designated ostA, was mapped at 1.2 min, close to pdxA. Transduction of ostA from a n-hexane-sensitive strain to a n-hexane-tolerant strain generated n-hexane-sensitive transductants. The sensitive transductant restored n-hexane-tolerance by transduction of ostA from a tolerant strain. Thus, the gene ostA is one of the genes that contributes to deciding the level of organic solvent tolerance in E. coli.