Stomatal movements are involved in elevated CO2 -mitigated high temperature stress in tomato.
Stomatal movements are involved in elevated CO2 -mitigated high temperature stress in tomato.
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DOI：
10.1111/ppl.12752
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发表时间：
2018-08
影响因子：
6.4
通讯作者：
Huan Zhang;Caizhe Pan;Shaohan Gu;Qiaomei Ma;Yiqing Zhang;Xin Li;K. Shi
中科院分区：
文献类型：
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作者：
Huan Zhang;Caizhe Pan;Shaohan Gu;Qiaomei Ma;Yiqing Zhang;Xin Li;K. Shi
热浪等气候变化经常影响植物生长，并对自然和农业生态系统构成越来越大的威胁。大气中二氧化碳的升高可以减轻热应激的负面影响，但其潜在机制仍不清楚。我们研究了在两种CO2浓度（400和800µmol mol-1）和两种温度（25和42°C）下生长的番茄幼苗，升高的CO2 （eCO2）和温度对过氧化氢（H2 O2）的产生和与耐热性相关的气孔运动特性的交互影响。eCO2改善了热应激的负面影响，同时增加了呼吸爆发氧化酶1 （RBOH1）转录本的数量、胞外H2 O2的积累和气孔孔径的减小。抑制RBOH1和慢型阴离子通道，抑制了eCO2诱导的气孔关闭，降低了eCO2增强的水分利用效率和耐热性。我们的研究结果表明，依赖rboh1的H2 O2积累参与了eCO2诱导的气孔关闭，这参与了在eCO2浓度下维持水分保持和热量损失之间的平衡。这一现象可能是eCO2诱导番茄耐热性的一个因素，这对于了解植物如何应对未来的极端气候和二氧化碳的变化至关重要。
Climate changes such as heat waves often affect plant growth and pose a growing threat to natural and agricultural ecosystems. Elevated atmospheric CO2 can mitigate the negative effects of heat stress, but the underlying mechanisms remain largely unclear. We examined the interactive effects of elevated CO2 (eCO2 ) and temperature on the generation of the hydrogen peroxide (H2 O2 ) and stomatal movement characteristics associated with heat tolerance in tomato seedlings grown under two CO2 concentrations (400 and 800 µmol mol-1 ) and two temperatures (25 and 42°C). eCO2 ameliorated the negative effects of heat stress, which was accompanied by greater amounts of RESPIRATORY BURST OXIDASE 1 (RBOH1) transcripts, apoplastic H2 O2 accumulation and decreased stomatal aperture. Silencing RBOH1 and SLOW-TYPE ANION CHANNEL, impeded eCO2 -induced stomatal closure and compromised the eCO2 -enhanced water use efficiency as well as the heat tolerance. Our findings suggest that RBOH1-dependent H2 O2 accumulation was involved in the eCO2 -induced stomatal closure, which participate in maintaining balance between water retention and heat loss under eCO2 concentrations. This phenomenon may be a contributor to eCO2 -induced heat tolerance in tomato, which will be critical for understanding how plants respond to both future climate extremes and changes in CO2 .