IMMUNOHISTOCHEMICAL DETECTION OF ESTROGEN-RECEPTORS IN PARAFFIN SECTIONS FROM PRIMARY AND METASTATIC BREAST-CANCER
IMMUNOHISTOCHEMICAL DETECTION OF ESTROGEN-RECEPTORS IN PARAFFIN SECTIONS FROM PRIMARY AND METASTATIC BREAST-CANCER
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DOI：
10.1016/s0344-0338(89)80286-9
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发表时间：
1989-12-01
影响因子：
2.8
通讯作者：
KAMBY, C
中科院分区：
文献类型：
--
作者：
RASMUSSEN, BB;KAMBY, C
随着抗雌激素受体（ER）单克隆抗体的可用性，有可能以化学方法证明ER的存在。一些抗体声称在福尔马林固定的石蜡包埋组织中具有反应性。我们评估了其中一种抗体D75的反应性，并在常规福尔马林固定、石蜡包埋的组织中发现了可接受的反应。将抗体应用于来自一组复发性乳腺癌患者的原发性和继发性肿瘤。转移病灶包括淋巴结转移、骨髓转移和肝转移。虽然41%的原发性肿瘤为ER阳性，但淋巴结、骨髓和肝转移的ER阳性率分别为35%、20%和17%。原发肿瘤和远处转移的ER状态之间的不一致性在骨髓转移的病例中为41%，在肝转移的病例中为44%。在大多数情况下，转移是从ER阳性原发肿瘤转移到ER阴性转移。结果支持ER阴性肿瘤细胞可能比分化更高的ER阳性肿瘤细胞更具侵袭性和更大的转移潜力的假设。
With the availability of monoclonal antibodies against the estrogen receptor (ER) it is possible to demonstrate the presence of ER immunohistochemically. Some of the antibodies are claimed to be reactive in formalin fixed, paraffin embedded tissue. We have evaluated the reactivity of one of these antibodies, D75 and found an acceptable reaction in routinely formalin fixed, paraffin embedded tissue. The antibody was applied to both primary and secondary tumors from a group of patients with recurrent breast cancer. The metastatic lesions consisted of lymph node metastases, bone marrow metastases, and liver metastases. While 41% of the primary tumors were ER-positive, this was only the case with 35%, 20%, and 17% of the lymph node, bone marrow, and liver metastases, respectively. The discordance between the ER-status of the primary tumor and the distant metastasis was 41% in cases of bone marrow metastases, and 44% in liver metastases. In most cases the shift was from an ER-positive primary tumor to an ER-negative metastasis. The results support the hypothesis that ER-negative tumor cells are probably more aggressive with a larger metastatic potential than the higher differentiated, ER-positive tumor cells.