ESTRONE AND ESTRADIOL LEVELS IN OVARIAN VENOUS BLOOD FROM RATS DURING ESTROUS CYCLE AND PREGNANCY
ESTRONE AND ESTRADIOL LEVELS IN OVARIAN VENOUS BLOOD FROM RATS DURING ESTROUS CYCLE AND PREGNANCY
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DOI：
10.1093/biolreprod/5.3.297
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发表时间：
1971-01-01
影响因子：
3.6
通讯作者：
SHAIKH, AA
中科院分区：
文献类型：
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作者：
SHAIKH, AA
使用葡聚糖涂层炭分离，通过放射免疫测定法测定大鼠卵巢静脉血浆中的雌酮和雌二醇（霍奇基斯和Knobil，未发表）。通过对血浆的醚提取物进行酚分配和柱色谱法（硅藻土微柱）进行类固醇的分离。在发情周期中，雌激素的分泌速率变化很大。动情间期E1、E2分泌率较低。然而，在发情前期的早晨，它们急剧上升。同一天21时至23时，水平再次显著下降，然后在发情期保持较低水平。E1和E2的分泌率在妊娠第3天早期与发情期观察到的值相似。在第4天，观察到E1和E2的分泌率显著增加，E1和E2的分泌高峰出现在1400 - 1600 hr之间。然后雌激素分泌再次下降到中间值，并有微小的波动，基本保持不变，直到妊娠结束时再次观察到增加。E2/E1比值在整个发情周期和妊娠期内变化范围为1~7。在最大分泌速率下观察到最大比率。
Estrone and estradiol measurements were made in rat ovarian vein plasma by radioimmunoassay using dextran coated charcoal for separation (Hotchkiss and Knobil, unpublished). Separation of the steroids was carried out by subjecting the ether extract of the plasma to phenolic partition and column chromatography (celite microcolumn). During the estrous cycle the estrogen secretion rate varied greatly. During diestrus the secretion rates of E1and E2were low. They had, however, risen sharply by the morning of proestrus. By 2100–2300 hr on the same day the levels again fell significantly, and then remained low during estrus. Secretion rates of E1and E2were similar early on Day 3 of pregnancy to the values seen at estrus. On Day 4 significant increases in secretion rates of both E1and E2were observed the peak secretion of E1and E2occurring between 1400–1600 hr. Estrogen secretion then dropped again to intermediate values and with minor fluctuations remained essentially unchanged until increases were again observed towards the end of pregnancy. The ratio of E2/E1varied from 1 to 7 throughout estrous cycle and pregnancy. The largest ratios were seen at the maximal rates of secretion.