Subgingival microbial profiles of Sudanese patients with aggressive periodontitis.
Subgingival microbial profiles of Sudanese patients with aggressive periodontitis.
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DOI：
10.1111/jre.12250
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发表时间：
2015-10
影响因子：
3.5
通讯作者：
Bolstad AI
中科院分区：
文献类型：
--
作者：
Elabdeen HR;Mustafa M;Hasturk H;Klepac-Ceraj V;Ali RW;Paster BJ;Van Dyke T;Bolstad AI
侵袭性牙周炎(AGP)在非洲人中很普遍，而且病程很快。虽然Aggregatibacter放线菌伴生菌的研究受到了极大的关注，但新的方法支持在AGP中存在一种复杂的龈下微生物区系。本研究的目的是用不同的分析方法绘制牙周下微生物区系图，并探索伴生放线菌和JP2克隆在一组患有AGP的苏丹人中的存在。由19名AGP患者组成的研究人群是从喀土穆科技大学(UST)寻求治疗的患者中招募的。15名健康受试者作为对照。使用人类口腔微生物鉴定微阵列分析菌斑样本272个分类群，使用DNA-DNA杂交棋盘分析26个牙周分类群。采用常规聚合酶链式反应(PCR)检测菌斑中伴生放线菌和JP2克隆。应用定量聚合酶链式反应(QPCR)对AGP患者唾液中伴生放线菌进行检测。尤里氏真细菌在AGP患者中的检出频率高于对照组，而结节埃希氏菌仅在AGP患者中发现。用口腔微生物鉴定芯片检测2例(12%)AGP患者的菌斑标本，用常规聚合酶链式反应(PCR)在5例(29%)AGP患者和6例(32%)AGP患者唾液标本中发现伴生放线菌。仅在一名患者中发现了JP2克隆。在这里研究的人群中，典型的牙周病原体在AGP中并不存在高含量。通常与AGP无关的真细菌种类经常在有疾病的个体中被检测到。使用具有不同灵敏度和检测水平的实验室方法可以识别微生物群落的差异。本研究的结果为进一步了解AGP奠定了基础。
Aggressive periodontitis (AgP) is prevalent and shows a rapid course in African individuals. Although a strong focus has been placed on Aggregatibacter actinomycetemcomitans, new methods support the existence of a complex subgingival microflora in AgP. The purpose of the present study was to map the subgingival microbiota as well as explore the presence of A. actinomycetemcomitans and the JP2 clone in a group of Sudanese individuals with AgP, using different analytical methods. A study population consisting of 19 patients with AgP was recruited from patients seeking treatment at University of Science and Technology (UST) in Khartoum. Fifteen healthy subjects were included as controls. Plaque samples were analyzed for 272 taxa using human oral microbe identification microarrays and for 26 periodontal taxa using DNA-DNA hybridization checkerboard. Conventional polymerase chain reaction (PCR) was applied for the detection of A. actinomycetemcomitans and the JP2 clone in plaque. Saliva from patients with AgP was analyzed using quantitative PCR (qPCR) for the detection of A. actinomycetemcomitans. Eubacterium yurii was detected more frequently in patients with AgP than in controls, and E. nodatum was found in patients with AgP only. A. actinomycetemcomitans was found in plaque samples of two (12%) patients by human oral microbe identification microarrays and in five (29%) patients with AgP by conventional PCR, as well as in six (32%) of the AgP saliva samples by qPCR. The JP2 clone was identified in only one patient. The classical periodontal pathogens were not present in high amounts in AgP in the population studied here. Species of Eubacterium, which are not typically associated with AgP, were often detected in individuals with disease. Using laboratory methods with different sensitivities and detection levels allowed identification of variances in microbial communities. The findings reported in this study provide a basis for the further understanding of AgP.