Association of polyamines in governing the chilling sensitivity of maize genotypes
Association of polyamines in governing the chilling sensitivity of maize genotypes
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DOI：
10.1007/s10725-008-9315-2
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发表时间：
2009-01-01
影响因子：
4.2
通讯作者：
Knapp, Allen
中科院分区：
文献类型：
--
作者：
Gao, Canhong;Hu, Jin;Knapp, Allen
低温胁迫是全球玉米生产的一个重要制约因素。本研究比较了玉米幼苗不同组织对低温的反应，并分析了低温胁迫下多胺含量的变化。研究了两种玉米（Zea mays L.）小麦自交系‘黄C’和‘Mo17’对低温敏感性不同。幼苗暴露于低温（5A度C）和冷害估计电导率（EC），丙二醛（MDA）浓度，腐胺（Put），亚精胺（Spd）和精胺（Spm）浓度的变化在根，中胚轴，胚芽鞘组织。低温胁迫后，玉米幼苗3种组织的膜透性（EC）、MDA含量和Put含量均增加，但根中Put含量没有增加。低温胁迫后，幼苗3个组织中Spd和Spm含量均下降。冷胁迫下黄C的EC值低于Mo17。此外，胚芽鞘组织的EC低于中胚轴在两个自交系。因此，中胚轴组织可以作为评价玉米耐冷性的指标。逐步回归分析表明，根系冷害与Spd浓度呈显著正相关，而中胚轴冷害主要与Put和Spd浓度相关。胚芽鞘中亚精胺和精胺含量与冷害相关。玉米耐冷性苗期多胺特性变化与回归分析相结合是评价玉米耐冷性的可靠方法。
Chilling stress is an important constraint of global production of maize. This study was undertaken to compare the chilling responses of different maize seedling tissues and to analyze changes in polyamines as a result of chilling stress. Reponses to chilling were characterized in two maize (Zea mays L.) inbred lines, 'HuangC' and 'Mo17', that putatively differ in chilling sensitivity. Seedlings were exposed to low temperature (5A degrees C) and chilling injury was estimated by electrical conductivity (EC), malonaldehyde (MDA) concentration, and by changes in putrescine (Put), spermidine (Spd) and spermine (Spm) concentrations in root, mesocotyl, and coleoptile tissues. Membrane permeability (as measured by EC), MDA concentrations and Put concentrations in the three tissue of maize seedlings increased after chilling stress, except for the Put concentration in roots. Spd and Spm concentrations in the three tissues of seedlings decreased after chilling stress. The EC for cold stressed tissues were lower in HuangC than Mo17. Also, the EC of coleoptile tissues were lower than for mesocotyl in both inbred lines. We suggest that mesocotyl tissue can be used to evaluate cold tolerance in maize. Stepwise regression analyses showed that chilling injury in roots was generally correlated with Spd concentration while in the mesocotyl injury was mainly correlated with Put and Spd concentrations. Spermidine and Spm concentrations in the coleoptile were correlated with chilling injury. Characteristics changes of polyamines in chill-tolerant maize seedling combined with regression analysis are a reliable method for evaluating chill tolerance in maize lines.