HTLV-1 viral RNA is detected rarely in plasma of HTLV-1 infected subjects
HTLV-1 viral RNA is detected rarely in plasma of HTLV-1 infected subjects
复制标题
DOI：
10.1002/jmv.24264
复制
发表时间：
2015-12-01
影响因子：
12.7
通讯作者：
Taylor, Graham P.
中科院分区：
文献类型：
--
作者：
Demontis, Maria Antonietta;Sadiq, Maaz Tahir;Taylor, Graham P.
感染 HTLV-1 的患者血浆被认为是非传染性的，但最近有报道在血浆中检测到 HTLV-1 基因组 RNA。该项目的目的是检测和定量血浆中的 HTLV-1 RNA，并评估其在诊断和预后方面的潜在价值。从 65 名 HTLV-1 感染者（27 名无症状携带者 [AC]）、17 名 HTLV-1 相关脊髓病 (HAM/TSP) 患者、14 名成人 T 细胞白血病/淋巴瘤 (ATLL) 患者、2 名同时感染 HIV 的受试者和 5 名其他 HTLV-1 相关疾病患者的 1ml 血浆中提取 RNA 并进行逆转录。使用引物进行 HTLV-1 特异性巢式 PCR 来扩增保守的 Tax 区域。所有样品均一式四份运行，巢式 PCR 产物通过凝胶电泳检测。在 18 名 (28%) 患者的血浆中检测到 HTLV-1 RNA，其拷贝数始终非常低（每毫升血浆 3-13 拷贝病毒 cDNA）。在血浆中检测到 HTLV-1 RNA 的患者和不可能检测到 HTLV-1 RNA 的患者之间，HTLV-1 前病毒载量的平均值没有差异。 HTLV-1基因组RNA可以在少数患者的血浆中检测到，但其水平或频率不足以用于临床或诊断。无论其他因素如何，HTLV-1 缺乏通过血浆传播的原因是 HTLV-1 病毒粒子很少存在。 J. Med。病毒。 87:2130-2134，2015 年。(c) 2015 年 Wiley 期刊公司。
Plasma of patients infected with HTLV-1 is considered non-infectious but detection of HTLV-1 genomic RNA in plasma has been recently reported. The aim of this project was to detect and quantify HTLV-1 RNA in plasma and assess its potential value in diagnosis and prognosis. RNA from 1ml of plasma from 65 subjects infected with HTLV-1 (27 asymptomatic carriers [AC]), 17 patients with HTLV-1-associated myelopathy (HAM/TSP), 14 with adult T-cell leukemia/lymphoma (ATLL), two co-infected with HIV, and five with other HTLV-1-associated disease, was extracted and reverse transcribed. HTLV-1 specific nested PCR was performed using primers to amplify the conserved Tax region. All samples were run in quadruplicate, nested PCR products were detected by gel electrophoresis. HTLV-1 RNA was detected in plasma from 18 (28%) patients, always at a very low copy number (3-13 copies viral cDNA per milliliter of plasma). Mean values of HTLV-1 proviral load did not differ between patients in whom HTLV-1 RNA was detected and patients in whom it was not possible to detect HTLV-1 RNA in plasma. HTLV-1 genomic RNA can be detected in the plasma of a minority of patients but not at a level or frequency to be useful clinically or diagnostically. Lack of transmission of HTLV-1 by plasma is due to the rare presence of HTLV-1 virions, regardless of any other factor. J. Med. Virol. 87:2130-2134, 2015. (c) 2015 Wiley Periodicals, Inc.