RCH1, A PROTEIN THAT SPECIFICALLY INTERACTS WITH THE RAG-1 RECOMBINATION-ACTIVATING PROTEIN
RCH1, A PROTEIN THAT SPECIFICALLY INTERACTS WITH THE RAG-1 RECOMBINATION-ACTIVATING PROTEIN
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DOI：
10.1073/pnas.91.13.6156
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发表时间：
1994-06-21
影响因子：
11.1
通讯作者：
OETTINGER, MA
中科院分区：
文献类型：
--
作者：
CUOMO, CA;KIRCH, SA;OETTINGER, MA
RAG1 和 R4G2 是淋巴特异性基因，它们共同诱导多种非淋巴细胞类型中的 V(D)J 重组酶活性。虽然不需要其他淋巴特异性因子来诱导重组，但具有更广泛表达模式的其他因子也参与了该反应。然而，这些因子均未被克隆，并且它们与 RAG 蛋白的关系尚不清楚。使用酵母双杂交测定，我们鉴定了 RCH1，这是一种编码分子量为 58,000 的蛋白质的基因，可与 RAG-1 特异性相互作用。预测的 Rch1 蛋白序列与酵母 SRP1（一种与核膜相关的蛋白）有 47% 的同一性。 Rch1 的截短形式保留了与 RAG-1 相互作用的能力，可降低 HeLa 细胞中的 V(D)J 重组活性。
RAG1 and R4G2 are lymphoid-specific genes that together induce V(D)J recombinase activity in a variety of nonlymphoid cell types. While no other lymphoid-specific factors are required to induce recombination, other factors with more widespread expression patterns have been implicated in the reaction. However, none of these factors have been cloned, and their relationship to the RAG proteins is unclear. Using the yeast two-hybrid assay, we have identified RCH1, a gene encoding a protein of molecular weight 58,000 that interacts specifically with RAG-1. The predicted Rch1 protein sequence is 47% identical to yeast SRP1, a protein associated with the nuclear envelope. A truncated form of Rch1, which retains the ability to interact with RAG-1, reduces V(D)J recombination activity in HeLa cells.