Regional sequencing collaboration reveals persistence of the T12 Vibrio cholerae O1 lineage in West Africa.
Regional sequencing collaboration reveals persistence of the T12 Vibrio cholerae O1 lineage in West Africa.
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作者：
Ekeng E;Tchatchouang S;Akenji B;Issaka BB;Akintayo I;Chukwu C;Dano ID;Melingui S;Ousmane S;Popoola MO;Nzouankeu A;Boum Y;Luquero F;Ahumibe A;Naidoo D;Azman A;Lessler J;Wohl S
尽管最近对非洲霍乱传播模式有了深入的了解，但对每隔几年就会爆发一次霍乱的西非的区域和地方动态仍然知之甚少。在受影响最严重的地区对霍乱弧菌进行协调的基因组监测，可能会揭示对霍乱控制重要的传播模式。在尼日利亚的一次区域测序研讨会上，我们对来自喀麦隆、尼日尔和尼日利亚的46株霍乱弧菌分离株（2018年至2019年为37株）进行了测序，以更好地了解在这三个国家传播的霍乱弧菌之间的关系。从这些分离株中，我们产生了44个完整的霍乱弧菌O1序列，并在1280个已发表的霍乱弧菌O1基因组的背景下进行分析。所有序列都属于T12霍乱弧菌第七次大流行谱系。对新生成和之前发表的霍乱弧菌基因组的系统发育分析表明，自2009年首次在西非地区观察到T12谱系以来，尽管在此期间没有霍乱报告，但T12谱系一直在西非境内持续传播。这一区域排序工作的结果为今后的区域协调监测工作提供了一个模式。该项目由比尔和梅林达·盖茨基金会OPP1195157提供资金。
Despite recent insights into cholera transmission patterns in Africa, regional and local dynamics in West Africa—where cholera outbreaks occur every few years—are still poorly understood. Coordinated genomic surveillance of Vibrio cholerae in the areas most affected may reveal transmission patterns important for cholera control. During a regional sequencing workshop in Nigeria, we sequenced 46 recent V. cholerae isolates from Cameroon, Niger, and Nigeria (37 from 2018 to 2019) to better understand the relationship between the V. cholerae bacterium circulating in these three countries. From these isolates, we generated 44 whole Vibrio cholerae O1 sequences and analyzed them in the context of 1280 published V. cholerae O1 genomes. All sequences belonged to the T12 V. cholerae seventh pandemic lineage. Phylogenetic analysis of newly generated and previously published V. cholerae genomes suggested that the T12 lineage has been continuously transmitted within West Africa since it was first observed in the region in 2009, despite lack of reported cholera in the intervening years. The results from this regional sequencing effort provide a model for future regionally coordinated surveillance efforts. Funding for this project was provided by Bill and Melinda Gates Foundation OPP1195157.