Roles of p38 MAPK, PKC and PI3-K in the signaling pathways of NADPH oxidase activation and phagocytosis in bovine polymorphonuclear leukocytes
Roles of p38 MAPK, PKC and PI3-K in the signaling pathways of NADPH oxidase activation and phagocytosis in bovine polymorphonuclear leukocytes
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DOI：
10.1016/s0014-5793(00)01167-4
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发表时间：
2000-02-11
期刊：
影响因子：
3.5
通讯作者：
Kuwabara, M
中科院分区：
文献类型：
--
作者：
Yamamori, T;Inanami, O;Kuwabara, M
用血清调理酵母聚糖 (sOZ) 刺激牛多形核白细胞 (PMN) 诱导 p38 丝裂原激活蛋白激酶 (MAPK)、蛋白激酶 C (PKC) 和磷脂酰肌醇 3-激酶 (PI3-K) 的激活，并且 sOZ 诱导的 O-2(-) 产生被其抑制剂显着减弱（p38 的 SB203580） MAPK、GF109203X（针对 PBC）和渥曼青霉素（针对 PD-K）。它们还导致 sOZ 诱导的 p47phox 磷酸化显着减弱。然而，流式细胞术分析表明，SB203580 和渥曼青霉素减弱了吞噬作用，但 GF109203X 促进了吞噬作用。结果表明，p38 MAPK 和 PI3-K 参与 NADPH 氧化酶激活（O-2(-) 产生）和吞噬作用的信号通路，而 PKC 单独参与 NADPH 氧化酶激活的信号通路，(C) 2000 欧洲生化协会联合会。
Stimulation of bovine polymorphonuclear leukocytes (PMN) with serum-opsonized zymosan (sOZ) induced the activation of p38 mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3-K) and sOZ-induced O-2(-) production was significantly attenuated by their inhibitors (SB203580 for p38 MAPK, GF109203X for PBC and wortmannin for PD-K). They caused significant attenuation of sOZ-induced phosphorylation of p47phox as well, Flow cytometric analysis, however, revealed that SB203580 and wortmannin attenuated phagocytosis, but GF109203X facilitated it. The results suggest that p38 MAPK and PI3-K participated in both signaling pathways of NADPH oxidase activation (O-2(-) production) and phagocytosis, and PKC participated in the signaling pathway of NADPH oxidase activation alone, (C) 2000 Federation of European Biochemical Societies.