PdPt nanoparticles anchored on the N-G with the integration of PANI nanohybrids as novel redox probe and catalyst for the detection of rs1801177.
PdPt nanoparticles anchored on the N-G with the integration of PANI nanohybrids as novel redox probe and catalyst for the detection of rs1801177.
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DOI：
10.1016/j.bios.2017.11.054
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发表时间：
2018-04
影响因子：
12.6
通讯作者：
Jing Wu;Junlin He;Chengli Zhang;Jun Chen;Y. Niu;Qiqi Yuan;Chao Yu
中科院分区：
文献类型：
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作者：
Jing Wu;Junlin He;Chengli Zhang;Jun Chen;Y. Niu;Qiqi Yuan;Chao Yu
脂蛋白脂酶(LPL)基因(Rs1801177)的单核苷酸多态性(SNP)与动脉粥样硬化的进展密切相关，威胁全球公众健康。本工作首次构建了一种相对简单、特异、超灵敏的电化学DNA生物传感器来检测rs1801177。用富勒烯(C60)/聚酰胺胺(PAMAM)/金(Au)纳米复合薄膜修饰玻碳电极。此外，还合成了氮掺杂石墨烯(N-G)/铂钯(PDPT)双金属纳米粒子/聚苯胺(PANI)纳米杂化材料，并将其用于标记信号探针。这些纳米杂化材料具有丰富的活性基团，高效的氧化还原和催化活性，可以作为氧化还原纳米探针的载体，而不需要对电活性物质和催化剂进行额外的修饰，从而有效地简化了操作步骤，缩短了分析时间。在纳米杂化材料催化过氧化氢的作用下，N-G/PDPT/PANI本身的检测信号可以显著增强，从而提高了检测的灵敏度。在最佳条件下，该传感器对rs1801177具有良好的检测性能，线性范围为10fM~10nM，检出限为3.33fM(S/N=3)。与可能的干扰物质相比，所提出的生物传感器对目标DNA具有很好的选择性。结果表明，该方法在临床研究中具有潜在的应用价值。
Single nucleotide polymorphism (SNP) in lipoprotein lipase (LPL) gene (rs1801177) is strongly associated with the increased progression of atherosclerosis, threatening global public health. In this work, a relatively simple, specific and ultrasensitive electrochemical DNA biosensor was constructed to detect rs1801177 for the first time. A glass carbon electrode was modified with fullerene (C60)/polyamidoamine (PAMAM)/gold (Au) nanoparticles nanocomposites film. In addition the nitrogen-doped graphene (N-G)/palladium platinum (PdPt) bimetallic nanoparticle/ polyaniline (PANI) nanohybrids were synthesised and used to label the signal probes. These nanohybrids have abundant active groups, and efficient redox and catalytic activity, allowing them to be used as the nanocarrier for a redox nanoprobe without the additional modification of electroactive substance and catalyst, which could effectively simplify the operation procedure and shorten the analysis time. With the catalysis of H2O2by nanohybrids, the detection signal of N-G/PdPt/PANI itself could be significantly enhanced, lead to the improvement of the sensitivity. Under optimal conditions, the electrochemical DNA biosensor exhibited desirable performance for the determination of rs1801177 with a wide linearity ranging from 10 fM to 10 nM and a relatively low detection limit of 3.33 fM (S/N=3). The proposed biosensor showed excellent selectivity to the target DNA compared to possible interfering substances. The results suggested that this method has potential applications in clinical research.