High-titre, cryostable cell-free varicella zoster virus
High-titre, cryostable cell-free varicella zoster virus
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DOI：
10.1007/s007050050364
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发表时间：
1998-01-01
影响因子：
2.7
通讯作者：
Mullarkey, J
中科院分区：
文献类型：
--
作者：
Harper, DR;Mathieu, N;Mullarkey, J
制备高滴度无细胞病毒的困难，以及这种病毒在冷冻和解冻时的不稳定性，严重阻碍了水痘带状疱疹病毒的研究。我们已经评估了一系列方案的使用，并证明在PBS-蔗糖-谷氨酸盐-血清缓冲液（PSGC）中冷冻后，可以回收19，000空斑形成单位/毫升的相对较高滴度。此外，我们已经证明，通过该方法获得的病毒在中和和抗病毒敏感性试验中给出了与从感染细胞新鲜制备的病毒相似的结果，从而允许在此类试验中使用高滴度、滴定的病毒储备液。
Work with varicella-zoster virus has been seriously hampered by the difficulty of preparing high-titre cell free virus, and by the instability of such virus when frozen and thawed. We have evaluated the use of a range of protocols and demonstrate that relatively high titres of 19,000 plaque forming units per millilitre may be recovered after freezing in PBS-sucrose-glutamate-serum buffer (PSGC). In addition, we have shown that the virus obtained by this method gives similar results in neutralisation and antiviral susceptibility assays to that freshly prepared from infected cells, allowing the use of high titre, titrated virus stocks in such assays.