Peritoneal B-2 cells comprise a distinct B-2 cell population with B-1b-like characteristics
Peritoneal B-2 cells comprise a distinct B-2 cell population with B-1b-like characteristics
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DOI：
10.1002/eji.200535142
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发表时间：
2006-05-01
影响因子：
5.4
通讯作者：
Rothstein, Thomas L.
中科院分区：
文献类型：
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作者：
Hastings, William D.;Tumang, Joseph R.;Rothstein, Thomas L.
B-1和B-2细胞是在发育、表面标志物表达、组织定位和功能方面不同的淋巴细胞群。虽然主要存在于小鼠的脾脏、淋巴结和循环中，但在腹膜腔中发现少量B-2细胞，该部位主要由B-1细胞聚集。在这里，我们的特点是腹膜B-2细胞，并确定它们的关系，B-1细胞。我们发现，腹膜B-2细胞似乎是中间脾B-2和腹膜B-1细胞的表面标志物表达的B220，CD 80，CD 43，几个标志物基因的表达，并在体外活力和IgM分泌。腹膜B-2细胞连续转移到严重联合免疫缺陷小鼠中导致获得类似于B-1 B细胞的表型，如Mac-1和CD 43上调以及CD 23下调所示。此外，过继转移的腹膜B-2细胞通过在受体小鼠中产生天然IgM来重现B-1细胞的功能。这些数据表明，腹膜B-2细胞表达B-1 B细胞的一些特征，并且这种相似性随着在腹膜腔中的时间增加而增加。
B-1 and B-2 cells are lymphocyte populations that differ in development, surface marker expression, tissue localization, and function. Though mainly found in the spleen, lymph nodes, and circulation of mice, small numbers of B-2 cells are found in the peritoneal cavity, a site predominantly populated by B-1 cells. Here, we characterized peritoneal B-2 cells, and determined their relationship to B-1 cells. We found that peritoneal B-2 cells appear to be intermediate between splenic B-2 and peritoneal B-1 cells in terms of surface marker expression of B220, CD80, and CD43, expression of several marker genes, and in vitro viability and IgM secretion. Adoptive transfer of peritoneal B-2 cells into severe combined immunodeficiency mice resulted in the acquisition of a phenotype reminiscent of B-1b cells, as shown by up-regulation of Mac-1 and CD43, and downregulation of CD23. Moreover, adoptively transferred peritoneal B-2 cells recapitulated B-1 cell function by producing natural IgM in recipient mice. These data suggest that peritoneal B-2 cells express some characteristics of B-1b cells and that this similarity increases with additional time in the peritoneal cavity.