INFLUENZA TYPE-A VIRUS NEURAMINIDASE DOES NOT PLAY A ROLE IN VIRAL ENTRY, REPLICATION, ASSEMBLY, OR BUDDING
INFLUENZA TYPE-A VIRUS NEURAMINIDASE DOES NOT PLAY A ROLE IN VIRAL ENTRY, REPLICATION, ASSEMBLY, OR BUDDING
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DOI：
10.1128/jvi.69.2.1099-1106.1995
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发表时间：
1995-02-01
影响因子：
5.4
通讯作者：
AIR, GM
中科院分区：
文献类型：
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作者：
LIU, CG;EICHELBERGER, MC;AIR, GM
我们用神经氨酸酶缺陷型流感病毒NWS-MVI，它是通过提供细菌神经氨酸酶(C.Liu和G.M.Air，病毒学194：403-407,1993)来筛选的，以确定神经氨酸酶在流感病毒复制中的作用。电子显微镜显示，NWS-MVI突变体的病毒粒子正常组装并形成与细胞表面相关的大聚集体。在没有神经氨酸酶的情况下生长的NWS-MVI病毒能够在没有添加神经氨酸酶的情况下在MDCK细胞中进行第二轮复制，表明这些聚集体中的病毒颗粒具有感染性，在细胞质空泡中也发现了病毒聚集体，当病毒感染的细胞在有铁蛋白的情况下孵育时，发现这些聚集体被铁蛋白标记，表明它们来自于细胞表面的摄取。当神经氨酸酶缺陷病毒滴鼻给C57BL/6小鼠时，从肺中恢复了低滴度的病毒，并产生了主要组织相容性复合体I类限制性细胞毒T细胞：这是细胞在体内感染的证据。在C57BL/6 nu/nu小鼠中，低水平的病毒至少持续了28天，但从未增加。这些结果表明，在细胞培养或小鼠体内，流感病毒的进入、复制或组装不需要神经氨酸酶。
We have used a neuraminidase-deficient influenza virus, NWS-Mvi, which,vas selected by supplying bacterial neuraminidase in the medium (C. Liu and G. M. Air, Virology 194:403-407, 1993), to define the role of neuraminidase in influenza virus replication, Electron microscopy showed that virions of the NWS-Mvi mutant assembled normally and formed large aggregates associated with cell surfaces. The NWS-MVi virus grown in the absence of neuraminidase was able to carry out a second round of replication in MDCK cells without added neuraminidase, indicating that the virus particles contained in these aggregates were infectious, Aggregates of virus were also found in cytoplasmic vacuoles, When virus-infected cells were incubated in the presence of ferritin, such aggregates were found to be labeled with ferritin, indicating that they are derived from uptake at the cell surface. When the neuraminidase-deficient virus was administered intranasally to C57BL/6 mice, low titers of virus were recovered from the lungs and major histocompatibility complex class I-restricted cytotoxic T cells were generated: evidence that cells were infected in vivo. In C57BL/6 nu/nu mice, the low level of virus persisted for at least 28 days but never increased, These results suggest that neuraminidase is not required for influenza virus entry, replication, or assembly in cell culture or in mice,