Spheroid-Based In Vitro Angiogenesis Model
Spheroid-Based In Vitro Angiogenesis Model
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DOI：
10.1007/978-1-4939-3628-1_11
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Korff, Thomas
中科院分区：
文献类型：
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作者：
Pfisterer, Larissa;Korff, Thomas
模拟毛细血管出芽的体外模型是研究肿瘤血管生成、发育血管形成和毛细血管系统病理生理重塑过程的重要工具。基于这一重点，Korff等人在1998年引入了内皮细胞（EC）球状体作为类似血管生成反应和发芽行为的三维体外模型[1]。因此，EC球状体能够产生毛细血管样芽，其相对接近血管中内皮细胞的生理和遗传程序化排列。由内皮细胞和平滑肌细胞组成的共培养球状体形成由平滑肌细胞和内皮细胞外层组成的球状核心，类似于较大血管的生理结构。在实践中，在圆底孔板或“悬滴”中培养限定数量的内皮细胞，以允许球状三维结构的形成和排列。随后，收获它们并包埋在胶原凝胶中，以允许源自每个球状体的内皮细胞芽生长。为了评估细胞因子或化合物的促血管生成或抗血管生成影响，确定芽的数量和长度。
In vitro models mimicking capillary sprouting are important tools to investigate the tumor angiogenesis, developmental blood vessel formation, and pathophysiological remodeling processes of the capillary system in the adult. With this focus, in 1998 Korff et al. introduced endothelial cell (EC) spheroids as a three-dimensional in vitro model resembling angiogenic responses and sprouting behavior [1]. As such, EC spheroids are capable of giving rise to capillary-like sprouts which are relatively close to the physiologically and genetically programmed arrangement of endothelial cells in vessels. Co-culture spheroids consisting of endothelial cells and smooth muscle cells form a spheroidal core composed of smooth muscle cells and an outer monolayer of endothelial cells, similar to the physiological architecture of larger blood vessels. In practise, a defined number of endothelial cells are cultured in a round-bottom well plate or in "hanging drops" to allow the formation and arrangement of the spheroidal three-dimensional structure. Subsequently, they are harvested and embedded in a collagen gel to allow outgrowth of endothelial cell sprouts originating from each spheroid. To evaluate the pro-or antiangiogenic impact of a cytokine or compound, the number and length of sprouts is determined.