In Vivo and In Vitro Feasibility Studies of Intraocular Use of Polyethylene Glycol-Based Synthetic Sealant to Close Retinal Breaks in Porcine and Rabbit Eyes
In Vivo and In Vitro Feasibility Studies of Intraocular Use of Polyethylene Glycol-Based Synthetic Sealant to Close Retinal Breaks in Porcine and Rabbit Eyes
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DOI：
10.1167/iovs.14-15349
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发表时间：
2015-07-01
影响因子：
4.4
通讯作者：
Oshika, Tetsuro
中科院分区：
文献类型：
--
作者：
Hoshi, Sujin;Okamoto, Fumiki;Oshika, Tetsuro
目的.可吸收的聚乙二醇基合成密封剂（PEG密封剂）在氙气照明下聚合，形成透明、柔韧且牢固粘附的水凝胶。评价PEG封闭剂的眼内生物相容性和对视网膜裂孔的封闭效果。在一项体外研究中，在玻璃体凝胶去除后，在猪眼杯中产生视网膜脱离伴撕裂。应用聚乙二醇基合成密封剂来覆盖撕裂，并使用40秒的氙气光进行聚合。通过将平衡盐溶液（BSS）强力喷射到视网膜裂孔上来测试视网膜粘附强度。将基于聚乙二醇的合成密封剂浸泡在BSS中，在37 ° C下孵育，并在72小时内定期测量pH。在一项体内研究中，将PEG密封剂注射到家兔左眼的玻璃体腔中。在注射前和注射后28天进行眼科检查，同时记录双侧ERG。摘除眼睛进行组织学评价。结果。在BSS中，PEG密封剂与视网膜的粘附良好。将BSS强力喷射到用PEG密封剂覆盖的视网膜裂孔上不会使视网膜脱离;没有PEG密封剂的视网膜裂孔立即脱离。含有PEG密封剂的BSS的pH在7.2和8.2之间。在28天的随访中，未观察到眼部炎症反应。注射前后的ERG均表现出典型的模式。组织学检查未发现任何异常或炎症。基于聚乙二醇的合成密封剂似乎可以有效地密封视网膜裂孔，并且对眼睛没有毒性。
PURPOSE. Absorbable polyethylene glycol-based synthetic sealant (PEG sealant) polymerizes under xenon illumination and forms a clear, flexible, and firmly adherent hydrogel. The intraocular biocompatibility of PEG sealant and efficacy for closing retinal breaks were evaluated.METHODS. In an in vitro study, retinal detachment with a tear was created in porcine eyecups after vitreous gel removal. Polyethylene glycol-based synthetic sealant was applied to cover the tear and polymerized with a 40-second application of xenon light. Retinal adhesion strength was tested by forcefully squirting balanced salt solution (BSS) onto the retinal tear. Polyethylene glycol-based synthetic sealant was soaked in the BSS, incubated at 37 degrees C, and the pH measured periodically over 72 hours. In an in vivo study, PEG sealant was injected into the vitreous cavity of the left eyes of rabbits. Ophthalmologic examinations were performed and bilateral ERGs were recorded simultaneously before and 28 days after injection. The eyes were enucleated for histological evaluation.RESULTS. Adhesion of PEG sealant to the retina was good in BSS. A forceful squirt of BSS onto the retinal tear covered with PEG sealant did not detach the retina; the retinal tear without PEG sealant detached immediately. The pH of the BSS containing PEG sealant was between 7.2 and 8.2. No inflammatory reaction was observed in the eyes throughout 28 days of follow-up. The ERGs recorded before and after injection showed typical patterns. Histological examinations did not reveal any abnormality or inflammation.CONCLUSIONS. Polyethylene glycol-based synthetic sealant appeared to effectively seal retinal breaks and was not toxic to the eye.