Polymerase chain reaction is more sensitive than viral culture and antigen testing for the detection of respiratory viruses in adults with hematological cancer and pneumonia
Polymerase chain reaction is more sensitive than viral culture and antigen testing for the detection of respiratory viruses in adults with hematological cancer and pneumonia
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DOI：
10.1086/338238
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发表时间：
2002-01-15
影响因子：
11.8
通讯作者：
van Loon, AM
中科院分区：
文献类型：
--
作者：
van Elden, LJR;van Kraaij, MGJ;van Loon, AM
我们回顾性分析了聚合酶链反应（PCR）检测43例血液肿瘤患者支气管肺泡灌洗（BAL）样本中呼吸道病毒感染的价值。此外，还采集了17份鼻咽喉拭子和29份血液样本。进行PCR检测副流感病毒1-3、呼吸道合胞病毒、鼻病毒、流感病毒A和B、肠道病毒和冠状病毒。BAL样本的病毒培养或抗原检测显示8例患者存在9种呼吸道病毒。应用PCR技术，在另外7例患者中又检出8种呼吸道病毒，使病毒检出率由19%提高到35%（P
We retrospectively analyzed the value of polymerase chain reaction (PCR) for the detection of respiratory viral infections in 43 patients with hematological cancer whose bronchoalveolar lavage (BAL) samples had been stored. In addition, 17 nose-throat (NT) swabs and 29 blood samples had been obtained. PCR was performed to detect parainfluenza viruses 1-3, respiratory syncytial virus, rhinovirus, influenza viruses A and B, enteroviruses, and coronaviruses. Viral cultures or antigen testing of BAL samples revealed 9 respiratory viruses in 8 patients. By use of PCR, 8 more respiratory viruses were detected in another 7 patients, increasing the rate of identification from 19% to 35% (P