Interleukin 2 regulates the activation of human basophils
Interleukin 2 regulates the activation of human basophils
复制标题
白介素 2 调节人类嗜碱性粒细胞的活化
DOI：
10.1016/j.cyto.2019.154934
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发表时间：
2020
期刊：
影响因子：
3.8
通讯作者：
Hui Wang
中科院分区：
文献类型：
--
作者：
Shuli Zhao;Yangyang Tang;Li Hong;Meizhen Xu;Shuai Pan;Kuiyang Zhen;Renxian Tang;Xiaoxiang Zhai;Zhixu Shi;Hui Wang
嗜碱性细胞是过敏性疾病和抗寄生虫免疫应答的重要效应细胞。许多激活因子，包括白细胞介素3 （IL-3）和IgE，已被确定在调节人类嗜碱性细胞表达介质，如组胺和白三烯C4 （LTC4）和细胞因子，包括IL-4和IL-13。人嗜碱性细胞表达高水平的IL-2受体。然而，IL-2通路在嗜碱性细胞中的作用尚不清楚。在这里，我们发现IL-2诱导人体外嗜碱性粒细胞表达多种炎症细胞因子和趋化因子，包括IL-5、IL-13、GM-CSF和CCL-17。IL-2的这种影响被上游调节器分析证实，该分析使用Ingenuity通路分析。值得注意的是，其中最受调节的细胞因子之一IL-5首次被发现是由IL-2而不是IL-3或抗ige诱导的人嗜碱性细胞。大疱性类天疱疮和湿疹皮肤标本的免疫荧光分析显示，皮肤病变中浸润性嗜碱性细胞广泛表达IL-5和GM-CSF。总之，我们的发现揭示了IL-2作为人类嗜碱性细胞的一种新的调节因子。这增加了一个新的层面来支持嗜碱性粒细胞在过敏性疾病中的重要性。
Basophils are important effector cells in allergic disorders and anti-parasitic immune response. A number of activators including interleukin 3 (IL-3) and IgE have been identified in the regulation of human basophils expressing mediators such as histamine and leukotriene C4 (LTC4) and cytokines, including IL-4 and IL-13. Human basophils express high levels of IL-2 receptors. However, the function of the IL-2 pathway in basophils remains unknown. Here, we identified that IL-2 induced the activation of human basophilsin vitroto express a variety of inflammatory cytokines and chemokines including IL-5, IL-13, GM-CSF and CCL-17. This effect by IL-2 is confirmed by an upstream regulator analysis using Ingenuity pathway analysis. Of note, one of the top regulated cytokines, IL-5, was for the first time identified to be induced by IL-2 in human basophils rather than IL-3 or anti-IgE. Immunofluorescence analysis of skin specimens from bullous pemphigoid and eczema revealed that infiltrating basophils in skin lesions widely expressed IL-5 and GM-CSF. Together, our findings reveal IL-2 as a novel regulator of human basophils. This adds a new layer to support the importance of basophils in allergic disorders.