Chromatin-remodelling factor BRG1 selectively activates a subset of interferon-α-inducible genes
Chromatin-remodelling factor BRG1 selectively activates a subset of interferon-α-inducible genes
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DOI：
10.1038/ncb855
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发表时间：
2002-10-01
影响因子：
21.3
通讯作者：
Wen, ZL
中科院分区：
文献类型：
--
作者：
Huang, M;Qian, F;Wen, ZL
与Brahma相关的基因1（BRG1）是依赖ATP的染色质重塑SWI2 - SNF2复合物的关键成分，并且涉及调控基因表达、细胞周期控制和肿瘤发生。在此我们报道BRG1与信号转导和转录激活因子2（STAT2）相互作用，STAT2是一种调控由干扰素 -α（IFN -α）介导的基因表达的转录因子。BRG1增强IFN -α诱导的9 - 27和IFI27的表达，但不增强所测试的其他四个靶基因的表达，这表明STAT2对不同靶基因的激活可能涉及不同的染色质修饰因子。我们的结果还表明，BRG1的募集和激活可能除了STAT2之外还需要其他顺式作用元件和反式作用元件。我们的研究将SWI2 - SNF2复合物与细胞因子诱导的基因表达调控联系起来，并可能确定BRG1介导的基因激活和肿瘤发生的分子机制。
Brahma-related gene 1 (BRG1) is a key component of the ATP-dependent chromatin-remodelling SWI2-SNF2 complex and has been implicated in regulating gene expression, cell-cycle control and tumorigenesis. Here we report that BRG1 interacts with signal transducer and activator of transcription 2 (STAT2)-a transcription factor that regulates gene expression mediated by interferon-alpha (IFN-alpha). BRG1 enhances the IFN-alpha-induced expression of 9-27 and IFI27 but not that of four other target genes tested, showing that the activation of different target genes by STAT2 may involve alternative chromatin modifiers. Our results also suggest that the recruitment and activation of BRG1 may require other cis-acting and trans-acting elements in addition to STAT2. Our study links the SWI2-SNF2 complex to the regulation of cytokine-induced gene expression and may identify a molecular mechanism of BRG1-mediated gene activation and tumorigenesis.