Mucilaginibacter psychrotolerans sp nov., isolated from peatlands
Mucilaginibacter psychrotolerans sp nov., isolated from peatlands
复制标题
从泥炭地中分离出的粘液杆菌 psychrotolerans sp nov.
DOI：
10.1099/ijsem.0.001560
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发表时间：
2017
影响因子：
2.8
通讯作者：
Zhou Yuguang
中科院分区：
文献类型：
--
作者：
Deng Yongcui;Shen Liang;Xu Baiqin;Liu Yongqin;Gu Zhengquan;Liu Hongcan;Zhou Yuguang
从青藏高原日干桥泥炭地分离到一株革兰氏阴性、杆状、无鞭毛、粉红色、耐寒的细菌NH 7 - 4 T。16 SrRNA基因序列与粘杆菌属的模式菌株的相似性为96.84 ~ 93.02%。 菌株NH 7 - 4 T的生长发生在0和30 °C之间和pH 5.0-9.0，最适生长温度为20 °C，生长的最适pH为约7.0。主要的类异戊二烯醌是MK-7。主要的细胞脂肪酸是特征3（C16：1ω 7 c和/或iso-C15：02-OH）、iso-C15：0、iso-C17：03-OH、C16：0、iso-C15：03-OH和C16：1ω5c。 NH 7 - 4 T菌株的主要极性脂质为磷脂酰乙醇胺。NH 7 - 4 T菌株在API 20 NE试纸条上不吸收任何底物，且最适生长温度较低，这使其区别于其他类型的粘杆菌属菌株。NH 7 - 4 T的DNA G+C含量为48.6mol%。基于系统发育、表型和化学分类学数据，菌株NH 7 - 4 T（=JCM 30607 T = CGMCC 1.14937T）代表Mucilaginibacter psychrotoleranssp.十一是建议。
A Gram-stain-negative, rod-shaped, non-flagellated, pink, cold-tolerant bacterial strain, NH7-4T, was isolated from the Riganqiao peatlands on the Tibetan Plateau. The 16S rRNA gene sequence of the novel isolate shared a pairwise similarity ranging from 96.84 to 93.02 % with type strains of species of the genusMucilaginibacter. Growth of strain NH7-4Toccurred between 0 and 30 °C and at pH 5.0–9.0, with an optimum growth temperature at 20 °C and an optimum pH for growth of approximately 7.0. The major isoprenoid quinone was MK-7. The major cellular fatty acids were summed feature 3 (C16 : 1ω7cand/or iso-C15 : 02-OH), iso-C15 : 0, iso-C17 : 03-OH, C16 : 0, iso-C15 : 03-OH and C16 : 1ω5c. The major polar lipid of strain NH7-4Twas phosphatidylethanolamine. Strain NH7-4Tdid not assimilate any substrates in API 20NE strips without low concentrations of yeast extract being present and had a lower optimal growth temperature, which distinguished it from other type strains of species of the genusMucilaginibacter. The DNA G+C content of strain NH7-4Twas 48.6 mol%. Based on phylogenetic, phenotypic and chemotaxonomic data, strain NH7-4T(=JCM 30607T=CGMCC1.14937T) represents a novel species of the genusMucilaginibacterfor which the nameMucilaginibacter psychrotoleranssp. nov. is proposed.