The Shaker Type Potassium Channel, GORK, Regulates Abscisic Acid Signaling in Arabidopsis
The Shaker Type Potassium Channel, GORK, Regulates Abscisic Acid Signaling in Arabidopsis
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DOI：
10.5423/ppj.oa.07.2019.0199
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发表时间：
2019-12-01
影响因子：
2.3
通讯作者：
Lee, Sung Chul
中科院分区：
文献类型：
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作者：
Lim, Chae Woo;Kim, Sang Hee;Lee, Sung Chul
植物对非生物胁迫的适应机制的进化对植物的生长发育至关重要。植物通过激活脱落酸（ABA）信号通路来适应逆境条件。ABA受体、进化枝A蛋白磷酸酶、SnRK2型激酶和SLAC1阴离子通道是ABA信号通路的重要组成部分。在这项研究中，我们报道了摇床型钾（K+）通道GORK调节植物对ABA和非生物胁迫的反应。我们的研究结果表明，PP2CA全长需要与GORK c端区相互作用。我们在gork中发现了一个显示aba低敏感性表型的功能等位基因缺失。突变体gork和pp2ca在种子萌发和幼苗生长中表现出相反的ABA反应。此外，gork突变体对NaCl和甘露醇处理具有耐受性，而pp2ca突变体对NaCl和甘露醇处理敏感。因此，我们的研究结果表明，GORK增强了对ABA的敏感性，并通过pp2ca介导的信号负向调节高盐度和渗透胁迫的机制。
Evolution of adaptive mechanisms to abiotic stress is essential for plant growth and development. Plants adapt to stress conditions by activating the abscisic acid (ABA) signaling pathway. It has been suggested that the ABA receptor, clade A protein phosphatase, SnRK2 type kinase, and SLAC1 anion channel are important components of the ABA signaling pathway. In this study, we report that the shaker type potassium (K+) channel, GORK, modulates plant responses to ABA and abiotic stresses. Our results indicate that the full length of PP2CA is needed to interact with the GORK C-terminal region. We identified a loss of function allele in gork that displayed ABA-hyposensitive phenotype. gork and pp2ca mutants showed opposite responses to ABA in seed germination and seedling growth. Additionally, gork mutant was tolerant to the NaCl and mannitol treatments, whereas pp2ca mutant was sensitive to the NaCl and mannitol treatments. Thus, our results indicate that GORK enhances the sensitivity to ABA and negatively regulates the mechanisms involved in high salinity and osmotic stresses via PP2CA-mediated signals.