The molecular organization of the Star/asteroid region, a region necessary for proper eye development in Drosophila melanogaster.
The molecular organization of the Star/asteroid region, a region necessary for proper eye development in Drosophila melanogaster.
复制标题
恒星/小行星区域的分子组织，这是果蝇眼睛正常发育所必需的区域。
作者：
Higson,TS;Tessiatore,JE;Bennett,SA;Derk,RC;Kotarski,MA
利用果蝇新染色体作为缺陷P元件的来源，用P元件转座子标签法克隆了果蝇（Drosophilamelanogaster）的星/星（S/ast）区。在每一个突变中，整合到该区域的元件是一个0.5 kb的元件，从一个区域近端的snand不是一个头对头元件从thesn基因座。通过Southern分析和限制性内切酶图谱分析，在分子图谱上定位了Sandast的自发突变和X射线诱导突变，突变是克隆区的大片段缺失或位于P元件插入附近的DNA断裂，从而引起突变。这两种突变都减少了3.4 kb RNA的稳态量。的分子数据，连同观察forSandast等位基因的表型相互作用，是一致的解释，即Sandast突变的病变内相同的基因或基因内的功能related. Keywords：果蝇，星星，小行星，P元素。
TheStar/asteroid(S/ast) region ofDrosophila melanogasterhas been cloned by P element transposon tagging using thesnwchromosome as a source of defective P elements. In each mutation examined, the element integrated into the region was a 0.5-kb element from a region proximal tosnand not one of the head-to-head elements from thesnlocus. Previously described spontaneous and X-ray induced mutations ofSandastwere located on the molecular map by Southern analysis and restriction endonuclease mapping of genomic clones.Smutations are either large deletions of the cloned region or DNA breaks located near the P element insertions that causeastmutations. BothSandastmutations reduce the steady-state amounts of a 3.4-kb RNA. The molecular data, together with the phenotypic interactions observed forSandastalleles, are consistent with the interpretation thatSandastmutations are lesions within the same gene or within genes that are functionally related.Key words:Drosophila,Star,asteroid, P elements.