LC3B globular structures correlate with survival in esophageal adenocarcinoma.
LC3B globular structures correlate with survival in esophageal adenocarcinoma.
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DOI：
10.1186/s12885-015-1574-5
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发表时间：
2015-08-12
期刊：
影响因子：
3.8
通讯作者：
McKenna SL
中科院分区：
文献类型：
--
作者：
El-Mashed S;O'Donovan TR;Kay EW;Abdallah AR;Cathcart MC;O'Sullivan J;O'Grady A;Reynolds J;O'Reilly S;O'Sullivan GC;McKenna SL
食管腺癌是西方世界实体瘤中发病率增长最快的。预后仍然很差，总体五年生存率低于 25%。只有有限数量的患者从化疗中受益，并且没有可以预测结果的生物标志物。先前的研究表明，自噬的诱导可以影响肿瘤细胞生物学的各个方面，包括化疗敏感性。本研究的目的是评估自噬标记物 (LC3B) 的表达是否与患者预后相关。对来自两个独立部位的食管腺癌肿瘤组织进行回顾性检查。在免疫组织化学分析之前，将来自 104 名新辅助治疗初治患者和 48 名新辅助治疗后患者的肿瘤组装成组织微阵列。 Kaplan-Meier 生存曲线和时序检验用于评估 LC3B 表达对生存的影响。 Cox 回归用于检查与临床危险因素的关联。发现 LC3B 表达的独特球状模式可以预测两个患者组的结果，无论治疗如何（p<<0.001）。多变量分析发现，这是不良预后的强有力的独立预测因子（p<<0.001）。 LC3B 的这种独特染色模式代表了可切除食管腺癌的新预后标志物。本文的在线版本 (doi:10.1186/s12885-015-1574-5) 包含补充材料，可供授权用户使用。
Esophageal adenocarcinoma has the fastest growing incidence of any solid tumor in the Western world. Prognosis remains poor with overall five-year survival rates under 25 %. Only a limited number of patients benefit from chemotherapy and there are no biomarkers that can predict outcome. Previous studies have indicated that induction of autophagy can influence various aspects of tumor cell biology, including chemosensitivity. The objective of this study was to assess whether expression of the autophagy marker (LC3B) correlated with patient outcome. Esophageal adenocarcinoma tumor tissue from two independent sites, was examined retrospectively. Tumors from 104 neoadjuvant naïve patients and 48 patients post neoadjuvant therapy were assembled into tissue microarrays prior to immunohistochemical analysis. Kaplan-Meier survival curves and log-rank tests were used to assess impact of LC3B expression on survival. Cox regression was used to examine association with clinical risk factors. A distinct globular pattern of LC3B expression was found to be predictive of outcome in both patient groups, irrespective of treatment (p < 0.001). Multivariate analysis found that this was a strong independent predictor of poor prognosis (p < 0.001). This distinctive staining pattern of LC3B represents a novel prognostic marker for resectable esophageal adenocarcinoma. The online version of this article (doi:10.1186/s12885-015-1574-5) contains supplementary material, which is available to authorized users.