Quantitative trait loci for cell wall components in recombinant inbred lines of maize (Zea mays L.) II:: leaf sheath tissue
Quantitative trait loci for cell wall components in recombinant inbred lines of maize (Zea mays L.) II:: leaf sheath tissue
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DOI：
10.1007/s00122-005-0175-0
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发表时间：
2006-02-01
影响因子：
5.4
通讯作者：
Coors, JG
中科院分区：
文献类型：
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作者：
Krakowsky, MD;Lee, M;Coors, JG
虽然玉米青贮饲料是动物生产操作中的重要饲料成分，但关于玉米中纤维和木质素浓度的遗传基础的信息很少，而纤维和木质素浓度与消化率呈负相关。纤维主要由纤维素、半纤维素和木质素组成，它们是植物细胞壁的主要成分。这些性状在玉米种质中的变异已有报道，但变异的来源以及不同组织中这些性状之间的关系还不清楚。在这项研究中，191个重组自交系B73（低-中等水平的细胞壁成分，CWC）×德811（高水平的CWC）进行了分析与CWC在叶鞘中的数量性状位点（QTL）。在1998年和1999年分别从两个地点和一个地点采集样品，并测定中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）和酸性洗涤木质素（ADL）。在所有10条染色体上都检测到QTL，大多数在组织特异性簇中，与同一组织内CWC的高基因型相关性一致。调整NDF的亚组分，ADF，揭示了大部分的NDF的遗传变异可能是由于ADF的变化。相同CWC在叶鞘和茎组织中的低至中等的基因型相关性表明，CWC的一些基因可能仅在某些组织中表达。本文中的许多QTL在其他群体中被检测到，并且一些QTL与细胞壁碳水化合物生物合成的候选基因连锁。
While maize silage is a significant feed component in animal production operations, little information is available on the genetic bases of fiber and lignin concentrations in maize, which are negatively correlated with digestibility. Fiber is composed largely of cellulose, hemicellulose and lignin, which are the primary components of plant cell walls. Variability for these traits in maize germplasm has been reported, but the sources of the variation and the relationships between these traits in different tissues are not well understood. In this study, 191 recombinant inbred lines of B73 (low-intermediate levels of cell wall components, CWCs) x De811 (high levels of CWCs) were analyzed for quantitative trait loci (QTL) associated with CWCs in the leaf sheath. Samples were harvested from plots at two locations in 1998 and one in 1999 and assayed for neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL). QTL were detected on all ten chromosomes, most in tissue specific clusters in concordance with the high genotypic correlations for CWCs within the same tissue. Adjustment of NDF for its subfraction, ADF, revealed that most of the genetic variation in NDF was probably due to variation in ADF. The low to moderate genotypic correlations for the same CWC across leaf sheath and stalk tissues indicate that some genes for CWCs may only be expressed in certain tissues. Many of the QTL herein were detected in other populations, and some are linked to candidate genes for cell wall carbohydrate biosynthesis.