A technique combining intracellular dye-marking, immunocytochemical identification and ultrastructural analysis of physiologically identified single neurons.
A technique combining intracellular dye-marking, immunocytochemical identification and ultrastructural analysis of physiologically identified single neurons.
复制标题
一种结合细胞内染料标记、免疫细胞化学鉴定和生理学鉴定的单个神经元的超微结构分析的技术。
DOI：
10.1016/0304-3940(82)90316-0
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发表时间：
1982
影响因子：
2.5
通讯作者：
Hayward,JN
中科院分区：
文献类型：
--
作者：
ReavesJr,TA;Cumming,R;Libber,MT;Hayward,JN
一种新的方法，产生一种不溶性的荧光黄注射神经元内的亲脂聚合物，使我们能够开发一种技术的电生理学特征，免疫细胞化学鉴定的单个神经元的超微结构检查。在这份初步报告中，我们研究了金鱼视前核中含有neurophysin的垂体投射神经元的光镜和电镜特征。
A new method which produces an insoluble osmophilic polymer within Lucifer Yellow-injected neurons has allowed us to develop a technique for the ultrastructural examination of electrophysiologically characterized, immunocytochemically identified single neurons. In this initial report, we examine the light- and electron-microscopic features of neurophysin-containing, pituitary-projecting neurons in the goldfish preoptic nucleus.